Hotspot

ycf1-rps15

Genome · 117526-117998 · 473 bp

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Pi 0.104

MarkerSeek score 71.20

Primer available yes

Label ycf1-rps15

Feature Metadata

label_name: ycf1-rps15
feature_type: igs
region: Genome
length_bp: 473
variable_sites: 239
pi: 0.104
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 71.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-rps15_p1	TGTATCCGCGATTGAAAATTTGA	TCAACTGATCCGGGGTTTTC	34.8	50.0	57.4	57.8	190-2998 bp	0.956522	60.3
ycf1-rps15_p2	TGTATCCGCGATTGAAAATTTGA	CAACTGATCCGGGGTTTTCT	34.8	50.0	57.4	57.8	189-2997 bp	0.956522	60.2
ycf1-rps15_p3	ATCCGCGATTGAAAATTTGA	TCAACTGATCCGGGGTTTTC	35.0	50.0	54.3	57.8	187-2995 bp	0.956522	58.8
ycf1-rps15_p4	ATCCGCGATTGAAAATTTGA	CAACTGATCCGGGGTTTTCT	35.0	50.0	54.3	57.8	186-2994 bp	0.956522	58.8
ycf1-rps15_p5	TGTATCCGCGATTGAAAATTTGA	ACTGATCCGGGGTTTTCTTC	34.8	50.0	57.4	57.5	187-2995 bp	0.956522	57.9