Hotspot

trnR-ACG-trnN-GUU

Genome · 110790-111414 · 625 bp

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Pi 0.0412

MarkerSeek score 75.20

Primer available yes

Label trnR-ACG-trnN-GUU

Feature Metadata

label_name: trnR-ACG-trnN-GUU
feature_type: igs
region: Genome
length_bp: 625
variable_sites: 104
pi: 0.0412
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 75.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-ACG-trnN-GUU_p1	CAGAGGATTAGAGCACGTGG	TTCCTCAGTAGCTCAGTGGT	55.0	50.0	57.8	57.7	648-849 bp	1.0	87.8
trnR-ACG-trnN-GUU_p2	CAGAGGATTAGAGCACGTGG	CCGATTGGTCGTAGGTTCAA	55.0	50.0	57.8	57.6	610-811 bp	0.956522	85.5
trnR-ACG-trnN-GUU_p3	CAGAGGATTAGAGCACGTGG	GTTCCTCAGTAGCTCAGTGG	55.0	55.0	57.8	57.3	649-850 bp	1.0	85.0
trnR-ACG-trnN-GUU_p4	GGCCTGTAGCTCAGAGGATT	TTCCTCAGTAGCTCAGTGGT	55.0	50.0	58.9	57.7	659-860 bp	1.0	82.5
trnR-ACG-trnN-GUU_p5	CAGAGGATTAGAGCACGTGG	CCTCAGTAGCTCAGTGGTAGA	55.0	52.4	57.8	58.0	646-847 bp	1.0	82.2