Hotspot

rrn5-trnR-ACG

Genome · 110393-110714 · 322 bp

Back to Caragana

Pi 0.0512
MarkerSeek score 76.00
Primer available yes
Label rrn5-trnR-ACG

Feature Metadata

label_name
rrn5-trnR-ACG
feature_type
igs
region
Genome
length_bp
322
variable_sites
64
pi
0.0512
unique_haplotype_count
18
primer_available
yes
alignment_reliability
0.99
markerseek_score
76.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rrn5-trnR-ACG_p1 GGTGACGATACTGTAGGGGA CCACGTGCTCTAATCCTCTG 55.0 55.0 57.9 57.8 287-522 bp 1.0 89.8
rrn5-trnR-ACG_p2 AATCCATCCCGAACTTGGTG CCACGTGCTCTAATCCTCTG 50.0 55.0 57.9 57.8 322-557 bp 1.0 89.2
rrn5-trnR-ACG_p3 ACCAATCCATCCCGAACTTG CCACGTGCTCTAATCCTCTG 50.0 55.0 57.9 57.8 325-560 bp 1.0 89.2
rrn5-trnR-ACG_p4 CAATCCATCCCGAACTTGGT CCACGTGCTCTAATCCTCTG 50.0 55.0 57.9 57.8 323-558 bp 1.0 89.2
rrn5-trnR-ACG_p5 CACCAATCCATCCCGAACTT CCACGTGCTCTAATCCTCTG 50.0 55.0 57.9 57.8 326-561 bp 1.0 89.2