Hotspot

rpl23-trnI-CAU

Genome · 89254-90604 · 1351 bp

Back to Caragana

Pi 0.233

MarkerSeek score 67.90

Primer available yes

Label rpl23-trnI-CAU

Feature Metadata

label_name: rpl23-trnI-CAU
feature_type: igs
region: Genome
length_bp: 1351
variable_sites: 402
pi: 0.233
unique_haplotype_count: 20
primer_available: yes
alignment_reliability: 0.38
markerseek_score: 67.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl23-trnI-CAU_p1	ATAGGTCGCCCTCTTCTACC	TCGGGAGTCCAAGAGTTTGA	55.0	50.0	58.0	58.3	934-2116 bp	0.782609	81.0
rpl23-trnI-CAU_p2	ATAGGTCGCCCTCTTCTACC	TGGAGAAGTCGTAGGTTCGA	55.0	50.0	58.0	57.8	580-1580 bp	0.173913	57.4
rpl23-trnI-CAU_p3	CACTTGACTGTCTACGGCTC	TGGAGAAGTCGTAGGTTCGA	55.0	50.0	58.0	57.8	819-1754 bp	0.130435	53.9
rpl23-trnI-CAU_p4	GACTGTCTACGGCTCCATTG	TGGAGAAGTCGTAGGTTCGA	55.0	50.0	58.1	57.8	814-1749 bp	0.130435	53.5
rpl23-trnI-CAU_p5	CCACAACGATGCTGTCCATA	TGGAGAAGTCGTAGGTTCGA	50.0	50.0	58.0	57.8	953-1804 bp	0.086957	52.0