Hotspot

rps12-clpP

Genome · 70496-71172 · 677 bp

Back to Caragana

Pi 0.174
MarkerSeek score 75.20
Primer available yes
Label rps12-clpP

Feature Metadata

label_name
rps12-clpP
feature_type
igs
region
Genome
length_bp
677
variable_sites
362
pi
0.174
unique_haplotype_count
19
primer_available
yes
alignment_reliability
0.88
markerseek_score
75.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps12-clpP_p1 ACCCTAGTACATGTCCCTCG AAGCCCACGGAATTGTTGAT 55.0 45.0 57.9 58.1 512-1620 bp 1.0 90.8
rps12-clpP_p2 AGAGCGGGAGATTTTGTGAC AAGCCCACGGAATTGTTGAT 50.0 45.0 57.9 58.1 473-1581 bp 1.0 90.6
rps12-clpP_p3 GAGCGGGAGATTTTGTGACA AAGCCCACGGAATTGTTGAT 50.0 45.0 58.2 58.1 472-1580 bp 1.0 89.9
rps12-clpP_p4 TGACATTTCGGATTGGCTGT AAGCCCACGGAATTGTTGAT 45.0 45.0 57.8 58.1 457-1565 bp 1.0 89.8
rps12-clpP_p5 TGTGACATTTCGGATTGGCT AAGCCCACGGAATTGTTGAT 45.0 45.0 57.8 58.1 459-1567 bp 1.0 89.8