Hotspot

psbK-trnQ-UUG

Genome · 56560-57234 · 675 bp

Back to Caragana

Pi 0.0329

MarkerSeek score 70.20

Primer available yes

Label psbK-trnQ-UUG

Feature Metadata

label_name: psbK-trnQ-UUG
feature_type: igs
region: Genome
length_bp: 675
variable_sites: 95
pi: 0.0329
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 70.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-trnQ-UUG_p1	ACTTGAATAAAGGGCAGAGTGA	GACCAAAACCCGTTGCCTTA	40.9	50.0	57.4	58.7	517-863 bp	1.0	65.4
psbK-trnQ-UUG_p2	ACTTGAATAAAGGGCAGAGTGA	CGAATAACGGGACCAAAACC	40.9	50.0	57.4	57.1	527-873 bp	1.0	63.6
psbK-trnQ-UUG_p3	ACTTGAATAAAGGGCAGAGTGA	ACCAAAACCCGTTGCCTTAC	40.9	50.0	57.4	59.0	516-862 bp	1.0	63.1
psbK-trnQ-UUG_p4	ACTTGAATAAAGGGCAGAGTGAA	GACCAAAACCCGTTGCCTTA	39.1	50.0	58.0	58.7	517-863 bp	1.0	62.4
psbK-trnQ-UUG_p5	ACTTGAATAAAGGGCAGAGTG	GACCAAAACCCGTTGCCTTA	42.9	50.0	55.9	58.7	517-863 bp	1.0	61.5