Hotspot

atpH-atpF

Genome · 50220-50900 · 681 bp

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Pi 0.0425

MarkerSeek score 83.80

Primer available yes

Label atpH-atpF

Feature Metadata

label_name: atpH-atpF
feature_type: igs
region: Genome
length_bp: 681
variable_sites: 94
pi: 0.0425
unique_haplotype_count: 18
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 83.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpF_p1	CCGGAAGCAGAGGACAAAAT	GATGGCCAGTAACTCAAGCA	50.0	50.0	58.2	57.9	769-848 bp	1.0	89.7
atpH-atpF_p2	CCGGAAGCAGAGGACAAAAT	CGGATGGCCAGTAACTCAAG	50.0	55.0	58.2	58.3	771-850 bp	1.0	87.9
atpH-atpF_p3	AGCAGAGGACAAAATACGGG	GATGGCCAGTAACTCAAGCA	50.0	50.0	57.6	57.9	764-843 bp	1.0	87.9
atpH-atpF_p4	CCGGAAGCAGAGGACAAAAT	TGGCCAGTAACTCAAGCAAA	50.0	45.0	58.2	57.6	767-846 bp	1.0	87.8
atpH-atpF_p5	TGGGCTGGTTGTAGCATTAG	GATGGCCAGTAACTCAAGCA	50.0	50.0	57.6	57.9	698-777 bp	1.0	87.7