primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnL-CAA-trnT-UGU_p1	trnL-CAA-trnT-UGU	1	ACCAATTTCGCCATATCCCC	GCGATGCTCTAACCTCTGAG	20	20	50.0	55.0	58.0	57.9	0.0	0.0	0.137364	14629	16162	1315-1771	1.000000	90.9
trnL-CAA-trnT-UGU_p2	trnL-CAA-trnT-UGU	2	TAGCGTCTACCAATTTCGCC	GCGATGCTCTAACCTCTGAG	20	20	50.0	55.0	58.1	57.9	0.0	0.0	0.202173	14629	16162	1323-1779	1.000000	90.4
trnL-CAA-trnT-UGU_p3	trnL-CAA-trnT-UGU	3	ACCAATTTCGCCATATCCCC	GGAATCGAACCGATGACCAT	20	20	50.0	50.0	58.0	57.8	0.0	15.5	0.245117	14629	16162	1347-1803	1.000000	90.0
trnL-CAA-trnT-UGU_p4	trnL-CAA-trnT-UGU	4	TAGCGTCTACCAATTTCGCC	GGAATCGAACCGATGACCAT	20	20	50.0	50.0	58.1	57.8	0.0	15.5	0.309926	14629	16162	1355-1811	1.000000	89.5
trnL-CAA-trnT-UGU_p5	trnL-CAA-trnT-UGU	5	CCCATTGTGAATTCCAGGGT	GCGATGCTCTAACCTCTGAG	20	20	50.0	55.0	57.8	57.9	2.3	0.0	0.369229	14629	16162	1408-1864	1.000000	89.0
trnG-GCC-psbZ_p1	trnG-GCC-psbZ	1	TACCATTCAACTATATCCGC	AGTGGGTATTCTTAATTCTCTCA	20	23	40.0	34.8	52.3	54.5	0.0	0.0	12.208911	26007	26645	526-708	1.000000	52.0
trnG-GCC-psbZ_p2	trnG-GCC-psbZ	2	TTACCATTCAACTATATCCGC	AGTGGGTATTCTTAATTCTCTCA	21	23	38.1	34.8	53.2	54.5	0.0	0.0	12.266359	26007	26645	527-709	1.000000	52.0
trnG-GCC-psbZ_p3	trnG-GCC-psbZ	3	TACCATTCAACTATATCCGC	AGTGGGTATTCTTAATTCTCTCATCT	20	26	40.0	34.6	52.3	57.4	0.0	0.0	12.283301	26007	26645	526-708	1.000000	52.0
trnG-GCC-psbZ_p4	trnG-GCC-psbZ	4	TTACCATTCAACTATATCCGC	AGTGGGTATTCTTAATTCTCTCATCT	21	26	38.1	34.6	53.2	57.4	0.0	0.0	12.340749	26007	26645	527-709	1.000000	52.0
trnG-GCC-psbZ_p5	trnG-GCC-psbZ	5	TTTACCATTCAACTATATCCGC	AGTGGGTATTCTTAATTCTCTCA	22	23	36.4	34.8	54.1	54.5	0.0	0.0	12.408369	26007	26645	528-710	1.000000	52.0
atpH-atpF_p1	atpH-atpF	1	CCGGAAGCAGAGGACAAAAT	GATGGCCAGTAACTCAAGCA	20	20	50.0	50.0	58.2	57.9	0.0	13.0	0.287475	50220	50900	769-848	1.000000	89.7
atpH-atpF_p2	atpH-atpF	2	CCGGAAGCAGAGGACAAAAT	CGGATGGCCAGTAACTCAAG	20	20	50.0	55.0	58.2	58.3	0.0	13.0	0.516963	50220	50900	771-850	1.000000	87.9
atpH-atpF_p3	atpH-atpF	3	AGCAGAGGACAAAATACGGG	GATGGCCAGTAACTCAAGCA	20	20	50.0	50.0	57.6	57.9	0.0	13.0	0.517112	50220	50900	764-843	1.000000	87.9
atpH-atpF_p4	atpH-atpF	4	CCGGAAGCAGAGGACAAAAT	TGGCCAGTAACTCAAGCAAA	20	20	50.0	45.0	58.2	57.6	0.0	0.0	0.530280	50220	50900	767-846	1.000000	87.8
atpH-atpF_p5	atpH-atpF	5	TGGGCTGGTTGTAGCATTAG	GATGGCCAGTAACTCAAGCA	20	20	50.0	50.0	57.6	57.9	0.0	13.0	0.532228	50220	50900	698-777	1.000000	87.7
trnG-UCC-trnS-GCU_p1	trnG-UCC-trnS-GCU	1	GGGGTCTATAGAACGAATCGC	AATCCGACGCTTTAGTCCAC	21	20	52.4	50.0	58.1	58.0	11.7	0.0	1.061978	55046	55546	484-618	1.000000	82.8
trnG-UCC-trnS-GCU_p2	trnG-UCC-trnS-GCU	2	ACGAATCGCACTTTTACCACT	AATCCGACGCTTTAGTCCAC	21	20	42.9	50.0	58.2	58.0	1.2	0.0	1.243225	55046	55546	472-606	1.000000	81.3
trnG-UCC-trnS-GCU_p3	trnG-UCC-trnS-GCU	3	GGGGTCTATAGAACGAATCGC	ACGGAAAGAGAGGGATTCGA	21	20	52.4	50.0	58.1	58.2	11.7	0.0	1.207355	55046	55546	538-672	1.000000	81.0
trnG-UCC-trnS-GCU_p4	trnG-UCC-trnS-GCU	4	GGGGTCTATAGAACGAATCGC	CAATCCGACGCTTTAGTCCA	21	20	52.4	50.0	58.1	58.0	11.7	0.0	1.060590	55046	55546	485-619	0.956522	79.5
trnG-UCC-trnS-GCU_p5	trnG-UCC-trnS-GCU	5	ACGAATCGCACTTTTACCACT	CAATCCGACGCTTTAGTCCA	21	20	42.9	50.0	58.2	58.0	1.2	0.0	1.241837	55046	55546	473-607	0.956522	78.2
psbK-trnQ-UUG_p1	psbK-trnQ-UUG	1	ACTTGAATAAAGGGCAGAGTGA	GACCAAAACCCGTTGCCTTA	22	20	40.9	50.0	57.4	58.7	0.0	0.0	3.320265	56560	57234	517-863	1.000000	65.4
psbK-trnQ-UUG_p2	psbK-trnQ-UUG	2	ACTTGAATAAAGGGCAGAGTGA	CGAATAACGGGACCAAAACC	22	20	40.9	50.0	57.4	57.1	0.0	0.0	3.546731	56560	57234	527-873	1.000000	63.6
psbK-trnQ-UUG_p3	psbK-trnQ-UUG	3	ACTTGAATAAAGGGCAGAGTGA	ACCAAAACCCGTTGCCTTAC	22	20	40.9	50.0	57.4	59.0	0.0	0.0	3.606415	56560	57234	516-862	1.000000	63.1
psbK-trnQ-UUG_p4	psbK-trnQ-UUG	4	ACTTGAATAAAGGGCAGAGTGAA	GACCAAAACCCGTTGCCTTA	23	20	39.1	50.0	58.0	58.7	0.0	0.0	3.693955	56560	57234	517-863	1.000000	62.4
psbK-trnQ-UUG_p5	psbK-trnQ-UUG	5	ACTTGAATAAAGGGCAGAGTG	GACCAAAACCCGTTGCCTTA	21	20	42.9	50.0	55.9	58.7	0.0	0.0	3.813955	56560	57234	517-863	1.000000	61.5
trnQ-UUG-accD_p1	trnQ-UUG-accD	1	GAGGTTCGAATCCTTCCGTC	TCCTGAAATAATCAACATTGCTGT	20	24	55.0	33.3	58.1	57.2	16.9	0.0	4.915225	57308	57919	654-713	0.956522	50.9
trnQ-UUG-accD_p2	trnQ-UUG-accD	2	GAGGTTCGAATCCTTCCGTC	CCTGAAATAATCAACATTGCTGT	20	23	55.0	34.8	58.1	55.8	16.9	0.0	5.280574	57308	57919	653-712	0.956522	50.3
trnQ-UUG-accD_p3	trnQ-UUG-accD	3	GAGGTTCGAATCCTTCCGTC	TGTTCCTGAAATAATCAACATTGCT	20	25	55.0	32.0	58.1	57.8	16.9	0.0	5.306950	57308	57919	657-716	0.956522	50.3
trnQ-UUG-accD_p4	trnQ-UUG-accD	4	GAGGTTCGAATCCTTCCGTC	TTCCTGAAATAATCAACATTGCTGT	20	25	55.0	32.0	58.1	57.8	16.9	0.0	5.306950	57308	57919	655-714	0.956522	50.3
trnQ-UUG-accD_p5	trnQ-UUG-accD	5	GAGGTTCGAATCCTTCCGTC	CCGTATGTTCCTGAAATAATCAACA	20	25	55.0	36.0	58.1	57.7	16.9	0.0	5.391348	57308	57919	662-721	0.956522	50.3
rps12-clpP_p1	rps12-clpP	1	ACCCTAGTACATGTCCCTCG	AAGCCCACGGAATTGTTGAT	20	20	55.0	45.0	57.9	58.1	0.0	0.0	0.145478	70496	71172	512-1620	1.000000	90.8
rps12-clpP_p2	rps12-clpP	2	AGAGCGGGAGATTTTGTGAC	AAGCCCACGGAATTGTTGAT	20	20	50.0	45.0	57.9	58.1	0.0	0.0	0.177249	70496	71172	473-1581	1.000000	90.6
rps12-clpP_p3	rps12-clpP	3	GAGCGGGAGATTTTGTGACA	AAGCCCACGGAATTGTTGAT	20	20	50.0	45.0	58.2	58.1	0.0	0.0	0.268234	70496	71172	472-1580	1.000000	89.9
rps12-clpP_p4	rps12-clpP	4	TGACATTTCGGATTGGCTGT	AAGCCCACGGAATTGTTGAT	20	20	45.0	45.0	57.8	58.1	0.0	0.0	0.275471	70496	71172	457-1565	1.000000	89.8
rps12-clpP_p5	rps12-clpP	5	TGTGACATTTCGGATTGGCT	AAGCCCACGGAATTGTTGAT	20	20	45.0	45.0	57.8	58.1	0.0	0.0	0.275471	70496	71172	459-1567	1.000000	89.8
clpP_p1	clpP	1	TCCAATCTAGCTGGGTCCAA	AACAGGGTCAGAGGGATCAA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.079378	71173	72505	2172-2172	0.043478	41.1
clpP_p2	clpP	2	TCCAATCTAGCTGGGTCCAA	ACAGGGTCAGAGGGATCAAA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.079378	71173	72505	2171-2171	0.043478	41.1
clpP_p3	clpP	3	TTCCAATCTAGCTGGGTCCA	AACAGGGTCAGAGGGATCAA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.079378	71173	72505	2173-2173	0.043478	41.1
clpP_p4	clpP	4	TTCCAATCTAGCTGGGTCCA	ACAGGGTCAGAGGGATCAAA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.079378	71173	72505	2172-2172	0.043478	41.1
clpP_p5	clpP	5	TCCAATCTAGCTGGGTCCAA	ACGAACATACCCTGTCTCCA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.101564	71173	72505	2204-2204	0.043478	40.9
rps19-rpl2_p1	rps19-rpl2	1	TCTCCTTTTGTGTTAAGCTTCT	TTGATTCTTCGTCGCCGTAG	22	20	36.4	50.0	55.6	58.1	0.0	0.0	4.514052	85370	87435	2173-2173	0.043478	5.6
rps19-rpl2_p2	rps19-rpl2	2	TCTCCTTTTGTGTTAAGCTTCT	TTGGGGTTATCCTGCACTTG	22	20	36.4	50.0	55.6	57.8	0.0	0.0	4.632572	85370	87435	2231-2231	0.043478	4.7
rps19-rpl2_p3	rps19-rpl2	3	TCTCCTTTTGTGTTAAGCTTCT	ATTTGATTCTTCGTCGCCGT	22	20	36.4	45.0	55.6	58.3	0.0	0.0	4.696185	85370	87435	2175-2175	0.043478	4.2
rps19-rpl2_p4	rps19-rpl2	4	TCTCCTTTTGTGTTAAGCTTCT	TTTGATTCTTCGTCGCCGTA	22	20	36.4	45.0	55.6	57.6	0.0	0.0	4.769095	85370	87435	2174-2174	0.043478	3.6
rps19-rpl2_p5	rps19-rpl2	5	TCTCCTTTTGTGTTAAGCTTCTT	TTGATTCTTCGTCGCCGTAG	23	20	34.8	50.0	56.3	58.1	0.0	0.0	4.784458	85370	87435	2173-2173	0.043478	3.5
rpl23-trnI-CAU_p1	rpl23-trnI-CAU	1	ATAGGTCGCCCTCTTCTACC	TCGGGAGTCCAAGAGTTTGA	20	20	55.0	50.0	58.0	58.3	0.0	12.3	0.289722	89254	90604	934-2116	0.782609	81.0
rpl23-trnI-CAU_p2	rpl23-trnI-CAU	2	ATAGGTCGCCCTCTTCTACC	TGGAGAAGTCGTAGGTTCGA	20	20	55.0	50.0	58.0	57.8	0.0	0.0	0.191371	89254	90604	580-1580	0.173913	57.4
rpl23-trnI-CAU_p3	rpl23-trnI-CAU	3	CACTTGACTGTCTACGGCTC	TGGAGAAGTCGTAGGTTCGA	20	20	55.0	50.0	58.0	57.8	9.5	0.0	0.205621	89254	90604	819-1754	0.130435	53.9
rpl23-trnI-CAU_p4	rpl23-trnI-CAU	4	GACTGTCTACGGCTCCATTG	TGGAGAAGTCGTAGGTTCGA	20	20	55.0	50.0	58.1	57.8	0.0	0.0	0.261699	89254	90604	814-1749	0.130435	53.5
rpl23-trnI-CAU_p5	rpl23-trnI-CAU	5	CCACAACGATGCTGTCCATA	TGGAGAAGTCGTAGGTTCGA	20	20	50.0	50.0	58.0	57.8	0.0	0.0	0.212358	89254	90604	953-1804	0.086957	52.0
rrn5-trnR-ACG_p1	rrn5-trnR-ACG	1	GGTGACGATACTGTAGGGGA	CCACGTGCTCTAATCCTCTG	20	20	55.0	55.0	57.9	57.8	0.0	10.9	0.278878	110393	110714	287-522	1.000000	89.8
rrn5-trnR-ACG_p2	rrn5-trnR-ACG	2	AATCCATCCCGAACTTGGTG	CCACGTGCTCTAATCCTCTG	20	20	50.0	55.0	57.9	57.8	0.0	10.9	0.344678	110393	110714	322-557	1.000000	89.2
rrn5-trnR-ACG_p3	rrn5-trnR-ACG	3	ACCAATCCATCCCGAACTTG	CCACGTGCTCTAATCCTCTG	20	20	50.0	55.0	57.9	57.8	0.0	10.9	0.344678	110393	110714	325-560	1.000000	89.2
rrn5-trnR-ACG_p4	rrn5-trnR-ACG	4	CAATCCATCCCGAACTTGGT	CCACGTGCTCTAATCCTCTG	20	20	50.0	55.0	57.9	57.8	0.0	10.9	0.344678	110393	110714	323-558	1.000000	89.2
rrn5-trnR-ACG_p5	rrn5-trnR-ACG	5	CACCAATCCATCCCGAACTT	CCACGTGCTCTAATCCTCTG	20	20	50.0	55.0	57.9	57.8	0.0	10.9	0.344678	110393	110714	326-561	1.000000	89.2
trnR-ACG-trnN-GUU_p1	trnR-ACG-trnN-GUU	1	CAGAGGATTAGAGCACGTGG	TTCCTCAGTAGCTCAGTGGT	20	20	55.0	50.0	57.8	57.7	18.9	0.0	0.526686	110790	111414	648-849	1.000000	87.8
trnR-ACG-trnN-GUU_p2	trnR-ACG-trnN-GUU	2	CAGAGGATTAGAGCACGTGG	CCGATTGGTCGTAGGTTCAA	20	20	55.0	50.0	57.8	57.6	18.9	0.0	0.589447	110790	111414	610-811	0.956522	85.5
trnR-ACG-trnN-GUU_p3	trnR-ACG-trnN-GUU	3	CAGAGGATTAGAGCACGTGG	GTTCCTCAGTAGCTCAGTGG	20	20	55.0	55.0	57.8	57.3	18.9	0.0	0.879733	110790	111414	649-850	1.000000	85.0
trnR-ACG-trnN-GUU_p4	trnR-ACG-trnN-GUU	4	GGCCTGTAGCTCAGAGGATT	TTCCTCAGTAGCTCAGTGGT	20	20	55.0	50.0	58.9	57.7	12.9	0.0	1.183436	110790	111414	659-860	1.000000	82.5
trnR-ACG-trnN-GUU_p5	trnR-ACG-trnN-GUU	5	CAGAGGATTAGAGCACGTGG	CCTCAGTAGCTCAGTGGTAGA	20	21	55.0	52.4	57.8	58.0	18.9	0.0	1.222019	110790	111414	646-847	1.000000	82.2
trnN-GUU-ycf1_p1	trnN-GUU-ycf1	1	ACCGAATAGCAGAGCAAGTG	CCTTTCTTCCATAACCCGGG	20	20	50.0	55.0	58.0	57.9	0.0	22.2	0.068518	111489	112248	913-1695	0.956522	89.7
trnN-GUU-ycf1_p2	trnN-GUU-ycf1	2	ACCGAATAGCAGAGCAAGTG	ACGGTAGAGTTAGGGCAGTT	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.081326	111489	112248	1043-1825	0.956522	89.6
trnN-GUU-ycf1_p3	trnN-GUU-ycf1	3	CGGGAGAATTGATGACTGCA	CCTTTCTTCCATAACCCGGG	20	20	50.0	55.0	58.0	57.9	0.0	22.2	0.081202	111489	112248	961-1605	0.913043	87.9
trnN-GUU-ycf1_p4	trnN-GUU-ycf1	4	CGGGAGAATTGATGACTGCA	ACGGTAGAGTTAGGGCAGTT	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.094010	111489	112248	1091-1735	0.913043	87.8
trnN-GUU-ycf1_p5	trnN-GUU-ycf1	5	ACCGACTAAAAGGGGAAGGA	CCTTTCTTCCATAACCCGGG	20	20	50.0	55.0	58.0	57.9	0.0	22.2	0.087219	111489	112248	1289-1950	0.739130	80.9
ycf1-rps15_p1	ycf1-rps15	1	TGTATCCGCGATTGAAAATTTGA	TCAACTGATCCGGGGTTTTC	23	20	34.8	50.0	57.4	57.8	8.2	0.0	3.747492	117526	117998	190-2998	0.956522	60.3
ycf1-rps15_p2	ycf1-rps15	2	TGTATCCGCGATTGAAAATTTGA	CAACTGATCCGGGGTTTTCT	23	20	34.8	50.0	57.4	57.8	8.2	0.0	3.753663	117526	117998	189-2997	0.956522	60.2
ycf1-rps15_p3	ycf1-rps15	3	ATCCGCGATTGAAAATTTGA	TCAACTGATCCGGGGTTTTC	20	20	35.0	50.0	54.3	57.8	8.2	0.0	3.928014	117526	117998	187-2995	0.956522	58.8
ycf1-rps15_p4	ycf1-rps15	4	ATCCGCGATTGAAAATTTGA	CAACTGATCCGGGGTTTTCT	20	20	35.0	50.0	54.3	57.8	8.2	0.0	3.934186	117526	117998	186-2994	0.956522	58.8
ycf1-rps15_p5	ycf1-rps15	5	TGTATCCGCGATTGAAAATTTGA	ACTGATCCGGGGTTTTCTTC	23	20	34.8	50.0	57.4	57.5	8.2	0.0	4.044965	117526	117998	187-2995	0.956522	57.9