Hotspot

rpl20

LSC · 71641-72105 · 465 bp

Back to Capsicum

Pi 0.0184

MarkerSeek score 57.90

Primer available yes

Label rpl20

Feature Metadata

label_name: rpl20
feature_type: gene
region: LSC
length_bp: 465
variable_sites: 55
pi: 0.0184
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20_p1	AGATTTGAGTATCGTGTCGT	TCGTCAAAGTTTCATTTATTCA	40.0	27.3	54.3	52.4	568-616 bp	1.0	51.5
rpl20_p2	AGATTTGAGTATCGTGTCGT	AGTTATTCGTCAAAGTTTCATT	40.0	27.3	54.3	52.3	574-622 bp	1.0	51.5
rpl20_p3	AGATTTGAGTATCGTGTCGT	TTCGTCAAAGTTTCATTTATTCA	40.0	26.1	54.3	53.2	569-617 bp	1.0	51.5
rpl20_p4	AGATTTGAGTATCGTGTCGT	AGTTATTCGTCAAAGTTTCATTT	40.0	26.1	54.3	53.2	574-622 bp	1.0	51.5
rpl20_p5	TCTAGATTTGAGTATCGTGTCGT	TCGTCAAAGTTTCATTTATTCA	39.1	27.3	56.9	52.4	571-619 bp	1.0	51.5