Hotspot

accD

LSC · 59910-61565 · 1656 bp

Back to Capsicum

Pi 0.00342

MarkerSeek score 55.70

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1656
variable_sites: 29
pi: 0.00342
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	CCCCAGATTCAGAACTTTTT	AAAATAATGGCCTTAGTGCT	40.0	35.0	53.3	52.8	1703-2021 bp	1.0	45.4
accD_p2	TCCTAGTGATTGGATTTCTATGCT	AAAATAATGGCCTTAGTGCT	37.5	35.0	57.2	52.8	1665-1971 bp	1.0	45.4
accD_p3	TCCTAGTGATTGGATTTCTATGC	AAAATAATGGCCTTAGTGCT	39.1	35.0	55.9	52.8	1665-1971 bp	1.0	45.4
accD_p4	CCTAGTGATTGGATTTCTATGCT	AAAATAATGGCCTTAGTGCT	39.1	35.0	55.9	52.8	1664-1970 bp	1.0	45.4
accD_p5	TCCTAGTGATTGGATTTCTATGCTT	AAAATAATGGCCTTAGTGCT	36.0	35.0	57.9	52.8	1665-1971 bp	1.0	45.4