Hotspot

rpl32-trnL

SSC · 115809-116678 · 870 bp

Back to Capsicum

Pi 0.00891

MarkerSeek score 51.40

Primer available yes

Label rpl32-trnL

Feature Metadata

label_name: rpl32-trnL
feature_type: igs
region: SSC
length_bp: 870
variable_sites: 33
pi: 0.00891
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL_p1	TCTACTGGGGAGTCAAAAGGT	TCCTAAGAGCAGCGTGTCTA	47.6	50.0	58.0	57.9	966 bp	0.066667	33.6
rpl32-trnL_p2	TCTACTGGGGAGTCAAAAGGT	TCGAACCGAGATGCATTAGC	47.6	50.0	58.0	58.1	992 bp	0.066667	33.4
rpl32-trnL_p3	TCTACTGGGGAGTCAAAAGGT	AGCAGCGTGTCTACCAATTT	47.6	45.0	58.0	57.8	959 bp	0.066667	33.1
rpl32-trnL_p4	TCTACTGGGGAGTCAAAAGGT	CCTAAGAGCAGCGTGTCTAC	47.6	55.0	58.0	57.8	965 bp	0.066667	33.0
rpl32-trnL_p5	TCTACTGGGGAGTCAAAAGGT	CTAAGAGCAGCGTGTCTACC	47.6	55.0	58.0	57.8	964 bp	0.066667	33.0