Hotspot

ndhF-rpl32

SSC · 115385-115637 · 253 bp

Back to Capsicum

Pi 0.01

MarkerSeek score 41.50

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 253
variable_sites: 19
pi: 0.01
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 41.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TGAAAGGTATGATCCACGCA	TGATCTCGAAGTGCGTTTTT	45.0	40.0	56.9	56.0	326-863 bp	1.0	60.8
ndhF-rpl32_p2	TGAAAGGTATGATCCACGCA	TTGATCTCGAAGTGCGTTTT	45.0	40.0	56.9	56.0	327-864 bp	1.0	60.8
ndhF-rpl32_p3	TGAAAGGTATGATCCACGCA	TTTGATCTCGAAGTGCGTTT	45.0	40.0	56.9	56.0	328-865 bp	1.0	60.8
ndhF-rpl32_p4	TGAAAGGTATGATCCACGCA	TTTTGATCTCGAAGTGCGTT	45.0	40.0	56.9	56.0	329-866 bp	1.0	60.8
ndhF-rpl32_p5	TGAAAGGTATGATCCACGCA	TTTTTGATCTCGAAGTGCGT	45.0	40.0	56.9	56.0	330-867 bp	1.0	60.8