Hotspot

accD-psaI

LSC · 61566-62325 · 760 bp

Back to Capsicum

Pi 0.00201

MarkerSeek score 52.50

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 760
variable_sites: 8
pi: 0.00201
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 52.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	AGTTATTTAAGCTCCACGCCT	AAGAAGCCATTGCAATTGCC	42.9	45.0	57.4	57.9	875-905 bp	1.0	70.5
accD-psaI_p2	TCCAGAAGGGTTTATTCGACC	AAGAAGCCATTGCAATTGCC	47.6	45.0	57.4	57.9	935-965 bp	1.0	70.3
accD-psaI_p3	CCAGAAGGGTTTATTCGACCT	AAGAAGCCATTGCAATTGCC	47.6	45.0	57.4	57.9	934-964 bp	1.0	70.2
accD-psaI_p4	TCGACCTAATTGTACCACGT	AAGAAGCCATTGCAATTGCC	45.0	45.0	56.3	57.9	920-950 bp	1.0	69.8
accD-psaI_p5	TCCAGAAGGGTTTATTCGACC	TGCAATTGCCGGAAAGACTA	47.6	45.0	57.4	57.8	925-955 bp	1.0	69.6