Hotspot

trnL-trnF

LSC · 49663-50255 · 593 bp

Back to Capsicum

Pi 0.00629

MarkerSeek score 58.80

Primer available yes

Label trnL-trnF

Feature Metadata

label_name: trnL-trnF
feature_type: igs
region: LSC
length_bp: 593
variable_sites: 20
pi: 0.00629
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-trnF_p1	ACATGTCAATACCGGCAACA	TTTGAACTGGTGACACGAGG	45.0	50.0	57.8	58.1	589-860 bp	1.0	84.2
trnL-trnF_p2	ACATGTCAATACCGGCAACA	GTGCCAGGAACCAGATTTGA	45.0	50.0	57.8	58.1	604-875 bp	1.0	83.9
trnL-trnF_p3	ACATGTCAATACCGGCAACA	AATCATGTGCCAGGAACCAG	45.0	50.0	57.8	58.2	610-881 bp	1.0	83.3
trnL-trnF_p4	ACATGTCAATACCGGCAACA	CATGTGCCAGGAACCAGATT	45.0	50.0	57.8	58.2	607-878 bp	1.0	83.3
trnL-trnF_p5	AATCGTGAGGGTTCAAGTCC	TTTGAACTGGTGACACGAGG	50.0	50.0	57.5	58.1	530-801 bp	1.0	82.1