Hotspot

trnE-trnT

LSC · 32286-32990 · 705 bp

Back to Capsicum

Pi 0.00245

MarkerSeek score 48.90

Primer available yes

Label trnE-trnT

Feature Metadata

label_name: trnE-trnT
feature_type: igs
region: LSC
length_bp: 705
variable_sites: 8
pi: 0.00245
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-trnT_p1	TTTCGTAGTACCCTACCCCC	GAACCGATGACTTACGCCTT	55.0	50.0	57.8	58.0	836-861 bp	1.0	80.9
trnE-trnT_p2	TTTCGTAGTACCCTACCCCC	CCATGGCGTTACTCTACCAC	55.0	55.0	57.8	58.1	815-840 bp	1.0	80.5
trnE-trnT_p3	TTTCGTAGTACCCTACCCCC	TGGCGTTACTCTACCACTGA	55.0	50.0	57.8	58.1	812-837 bp	1.0	80.4
trnE-trnT_p4	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	55.0	50.0	58.3	58.0	798-823 bp	1.0	80.2
trnE-trnT_p5	TTTCGTAGTACCCTACCCCC	CCGATGACTTACGCCTTACC	55.0	55.0	57.8	58.2	833-858 bp	1.0	79.8