Hotspot

rps16-trnQ

LSC · 6235-7506 · 1272 bp

Back to Capsicum

Pi 0.00672

MarkerSeek score 57.90

Primer available yes

Label rps16-trnQ

Feature Metadata

label_name: rps16-trnQ
feature_type: igs
region: LSC
length_bp: 1272
variable_sites: 41
pi: 0.00672
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ_p1	AACGGATCGTGTCCTTCAAG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.9	58.1	1305-1429 bp	1.0	85.1
rps16-trnQ_p2	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1288-1412 bp	1.0	84.7
rps16-trnQ_p3	CAACGGATCGTGTCCTTCAA	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	1306-1430 bp	1.0	84.1
rps16-trnQ_p4	CGGATCGTGTCCTTCAAGTC	GAGGTTCGAATCCTTCCGTC	55.0	55.0	58.4	58.1	1303-1427 bp	1.0	82.8
rps16-trnQ_p5	GGATCGTGTCCTTCAAGTCG	GAGGTTCGAATCCTTCCGTC	55.0	55.0	58.4	58.1	1302-1426 bp	1.0	82.8