primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-psbA_p1	trnH-psbA	1	GATCCACTTGGCTACATCCG	CCGTGCTAACCTTGGTATGG	20	20	55.0	55.0	58.1	58.3	0.0	0.0	0.465225	97	564	580-595	1.000000	79.6
trnH-psbA_p2	trnH-psbA	2	CAATCCACTGCCTTGATCCA	CCGTGCTAACCTTGGTATGG	20	20	50.0	55.0	57.9	58.3	0.0	0.0	0.469587	97	564	594-609	1.000000	79.4
trnH-psbA_p3	trnH-psbA	3	AATCCACTGCCTTGATCCAC	CCGTGCTAACCTTGGTATGG	20	20	50.0	55.0	57.9	58.3	0.0	0.0	0.471094	97	564	593-608	1.000000	79.4
trnH-psbA_p4	trnH-psbA	4	ACAATCCACTGCCTTGATCC	CCGTGCTAACCTTGGTATGG	20	20	50.0	55.0	57.9	58.3	0.0	0.0	0.471094	97	564	595-610	1.000000	79.4
trnH-psbA_p5	trnH-psbA	5	GATCCACTTGGCTACATCCG	ACCGTGCTAACCTTGGTATG	20	20	55.0	50.0	58.1	57.6	0.0	0.0	0.525510	97	564	581-596	1.000000	79.1
rps16-trnQ_p1	rps16-trnQ	1	AACGGATCGTGTCCTTCAAG	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.9	58.1	7.8	16.9	0.156387	6235	7506	1305-1429	1.000000	85.1
rps16-trnQ_p2	rps16-trnQ	2	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	6235	7506	1288-1412	1.000000	84.7
rps16-trnQ_p3	rps16-trnQ	3	CAACGGATCGTGTCCTTCAA	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.2	58.1	7.8	16.9	0.285346	6235	7506	1306-1430	1.000000	84.1
rps16-trnQ_p4	rps16-trnQ	4	CGGATCGTGTCCTTCAAGTC	GAGGTTCGAATCCTTCCGTC	20	20	55.0	55.0	58.4	58.1	7.8	16.9	0.445953	6235	7506	1303-1427	1.000000	82.8
rps16-trnQ_p5	rps16-trnQ	5	GGATCGTGTCCTTCAAGTCG	GAGGTTCGAATCCTTCCGTC	20	20	55.0	55.0	58.4	58.1	2.9	16.9	0.445953	6235	7506	1302-1426	1.000000	82.8
atpH-atpI_p1	atpH-atpI	1	ATAACGGAAGCGGCAGAAAT	TACCTGTCATGCTCCTTGGA	20	20	45.0	50.0	58.0	58.0	1.4	0.0	0.087178	14023	15206	1320-1328	1.000000	82.1
atpH-atpI_p2	atpH-atpI	2	TACCCTCTACAGCTTGACCC	TACCTGTCATGCTCCTTGGA	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.186809	14023	15206	1403-1411	1.000000	81.2
atpH-atpI_p3	atpH-atpI	3	GCGATACCCTCTACAGCTTG	TACCTGTCATGCTCCTTGGA	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.190326	14023	15206	1407-1415	1.000000	81.2
atpH-atpI_p4	atpH-atpI	4	AATAACGGAAGCGGCAGAAA	TACCTGTCATGCTCCTTGGA	20	20	45.0	50.0	58.2	58.0	1.4	0.0	0.232716	14023	15206	1321-1329	1.000000	81.0
atpH-atpI_p5	atpH-atpI	5	GCCAATCCAGCAGCAATAAC	TACCTGTCATGCTCCTTGGA	20	20	50.0	50.0	57.8	58.0	0.0	0.0	0.263178	14023	15206	1335-1343	1.000000	80.7
trnE-trnT_p1	trnE-trnT	1	TTTCGTAGTACCCTACCCCC	GAACCGATGACTTACGCCTT	20	20	55.0	50.0	57.8	58.0	0.0	0.0	0.165150	32286	32990	836-861	1.000000	80.9
trnE-trnT_p2	trnE-trnT	2	TTTCGTAGTACCCTACCCCC	CCATGGCGTTACTCTACCAC	20	20	55.0	55.0	57.8	58.1	0.0	0.0	0.223247	32286	32990	815-840	1.000000	80.5
trnE-trnT_p3	trnE-trnT	3	TTTCGTAGTACCCTACCCCC	TGGCGTTACTCTACCACTGA	20	20	55.0	50.0	57.8	58.1	0.0	0.0	0.245401	32286	32990	812-837	1.000000	80.4
trnE-trnT_p4	trnE-trnT	4	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	20	20	55.0	50.0	58.3	58.0	0.0	0.0	0.269359	32286	32990	798-823	1.000000	80.2
trnE-trnT_p5	trnE-trnT	5	TTTCGTAGTACCCTACCCCC	CCGATGACTTACGCCTTACC	20	20	55.0	55.0	57.8	58.2	0.0	0.0	0.310083	32286	32990	833-858	1.000000	79.8
psaA-ycf3_p1	psaA-ycf3	1	GCCCATTCCTCGAAAGAAGT	ATCAAGCCGCTGAGTATTGG	20	20	50.0	50.0	57.9	58.0	4.1	0.0	0.156301	43260	44099	926-1032	1.000000	84.9
psaA-ycf3_p2	psaA-ycf3	2	GCCCATTCCTCGAAAGAAGT	GGAAACAGGCTATAGCGCTT	20	20	50.0	50.0	57.9	58.0	4.1	18.7	0.160416	43260	44099	908-1014	1.000000	84.9
psaA-ycf3_p3	psaA-ycf3	3	GCCCATTCCTCGAAAGAAGT	CAGAGGCTTGGTTCGATCAA	20	20	50.0	50.0	57.9	57.9	4.1	0.0	0.214484	43260	44099	941-1047	1.000000	84.4
psaA-ycf3_p4	psaA-ycf3	4	GCCCATTCCTCGAAAGAAGT	CGATCAAGCCGCTGAGTATT	20	20	50.0	50.0	57.9	58.1	4.1	0.0	0.257251	43260	44099	928-1034	1.000000	84.1
psaA-ycf3_p5	psaA-ycf3	5	GCCCATTCCTCGAAAGAAGT	AGCCGCTGAGTATTGGAAAC	20	20	50.0	50.0	57.9	58.3	4.1	0.0	0.376276	43260	44099	922-1028	1.000000	83.2
rps4-trnT_p1	rps4-trnT	1	GATCATTTCCGGTCCTAGGC	CTCAGAGGTTAGAGCATCGC	20	20	55.0	55.0	57.8	57.9	3.0	0.0	0.304081	47998	48373	533-538	1.000000	83.1
rps4-trnT_p2	rps4-trnT	2	ATCATTTCCGGTCCTAGGCT	CTCAGAGGTTAGAGCATCGC	20	20	50.0	55.0	58.2	57.9	3.0	0.0	0.324926	47998	48373	532-537	1.000000	83.0
rps4-trnT_p3	rps4-trnT	3	GATCATTTCCGGTCCTAGGC	AGATTTCTGTGATGTGGGCC	20	20	55.0	50.0	57.8	57.9	3.0	0.0	0.306130	47998	48373	560-565	1.000000	82.8
rps4-trnT_p4	rps4-trnT	4	ATCATTTCCGGTCCTAGGCT	AGATTTCTGTGATGTGGGCC	20	20	50.0	50.0	58.2	57.9	3.0	0.0	0.326975	47998	48373	559-564	1.000000	82.7
rps4-trnT_p5	rps4-trnT	5	GATCATTTCCGGTCCTAGGC	ATGGTCATCGGTTCGATTCC	20	20	55.0	50.0	57.8	57.8	3.0	0.0	0.411834	47998	48373	501-506	1.000000	82.6
trnL-trnF_p1	trnL-trnF	1	ACATGTCAATACCGGCAACA	TTTGAACTGGTGACACGAGG	20	20	45.0	50.0	57.8	58.1	0.0	20.1	0.244559	49663	50255	589-860	1.000000	84.2
trnL-trnF_p2	trnL-trnF	2	ACATGTCAATACCGGCAACA	GTGCCAGGAACCAGATTTGA	20	20	45.0	50.0	57.8	58.1	0.0	0.0	0.282964	49663	50255	604-875	1.000000	83.9
trnL-trnF_p3	trnL-trnF	3	ACATGTCAATACCGGCAACA	AATCATGTGCCAGGAACCAG	20	20	45.0	50.0	57.8	58.2	0.0	0.0	0.349295	49663	50255	610-881	1.000000	83.3
trnL-trnF_p4	trnL-trnF	4	ACATGTCAATACCGGCAACA	CATGTGCCAGGAACCAGATT	20	20	45.0	50.0	57.8	58.2	0.0	0.0	0.349295	49663	50255	607-878	1.000000	83.3
trnL-trnF_p5	trnL-trnF	5	AATCGTGAGGGTTCAAGTCC	TTTGAACTGGTGACACGAGG	20	20	50.0	50.0	57.5	58.1	0.0	20.1	0.526305	49663	50255	530-801	1.000000	82.1
trnF-ndhJ_p1	trnF-ndhJ	1	AATAGCCGGGATAGCTCAGT	AGTTGGATAGGATGGCCCTT	20	20	50.0	50.0	58.0	58.1	2.2	0.0	0.104043	50329	50999	816-820	1.000000	81.4
trnF-ndhJ_p2	trnF-ndhJ	2	ATAGCCGGGATAGCTCAGTT	AGTTGGATAGGATGGCCCTT	20	20	50.0	50.0	58.0	58.1	2.2	0.0	0.104043	50329	50999	815-819	1.000000	81.4
trnF-ndhJ_p3	trnF-ndhJ	3	CCTCGTGTCACCAGTTCAAA	AGTTGGATAGGATGGCCCTT	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.139034	50329	50999	773-777	1.000000	81.3
trnF-ndhJ_p4	trnF-ndhJ	4	GGTTACCAGTCCCAACCTTG	AGTTGGATAGGATGGCCCTT	20	20	55.0	50.0	58.1	58.1	0.0	0.0	0.181501	50329	50999	855-859	1.000000	80.7
trnF-ndhJ_p5	trnF-ndhJ	5	ATAGCCGGGATAGCTCAGTT	ATCATCCACGCTTGAAACGT	20	20	50.0	45.0	58.0	58.2	2.2	0.0	0.208494	50329	50999	850-854	1.000000	80.5
ndhC-trnV_p1	ndhC-trnV	1	TTGCTAATCGGGGCTAACAC	CGGTTCAAGTCCGTATAGCC	20	20	50.0	55.0	58.0	58.2	0.0	0.0	0.183584	52679	53814	1251-1273	1.000000	82.8
ndhC-trnV_p2	ndhC-trnV	2	TTGCTAATCGGGGCTAACAC	GGTTCAAGTCCGTATAGCCC	20	20	50.0	55.0	58.0	57.8	0.0	0.0	0.268526	52679	53814	1250-1272	1.000000	82.1
ndhC-trnV_p3	ndhC-trnV	3	GCTAATCGGGGCTAACACTC	CGGTTCAAGTCCGTATAGCC	20	20	55.0	55.0	58.1	58.2	0.0	0.0	0.283737	52679	53814	1249-1271	1.000000	82.0
ndhC-trnV_p4	ndhC-trnV	4	GCTAATCGGGGCTAACACTC	GGTTCAAGTCCGTATAGCCC	20	20	55.0	55.0	58.1	57.8	0.0	0.0	0.368679	52679	53814	1248-1270	1.000000	81.3
ndhC-trnV_p5	ndhC-trnV	5	CCTTTGCTAATCGGGGCTAA	CGGTTCAAGTCCGTATAGCC	20	20	50.0	55.0	57.7	58.2	0.0	0.0	0.492750	52679	53814	1254-1276	1.000000	80.3
accD_p1	accD	1	CCCCAGATTCAGAACTTTTT	AAAATAATGGCCTTAGTGCT	20	20	40.0	35.0	53.3	52.8	0.0	3.3	9.889310	59910	61565	1703-2021	1.000000	45.4
accD_p2	accD	2	TCCTAGTGATTGGATTTCTATGCT	AAAATAATGGCCTTAGTGCT	24	20	37.5	35.0	57.2	52.8	0.0	3.3	9.983924	59910	61565	1665-1971	1.000000	45.4
accD_p3	accD	3	TCCTAGTGATTGGATTTCTATGC	AAAATAATGGCCTTAGTGCT	23	20	39.1	35.0	55.9	52.8	0.0	3.3	10.354517	59910	61565	1665-1971	1.000000	45.4
accD_p4	accD	4	CCTAGTGATTGGATTTCTATGCT	AAAATAATGGCCTTAGTGCT	23	20	39.1	35.0	55.9	52.8	0.0	3.3	10.364360	59910	61565	1664-1970	1.000000	45.4
accD_p5	accD	5	TCCTAGTGATTGGATTTCTATGCTT	AAAATAATGGCCTTAGTGCT	25	20	36.0	35.0	57.9	52.8	0.0	3.3	10.371769	59910	61565	1665-1971	1.000000	45.4
accD-psaI_p1	accD-psaI	1	AGTTATTTAAGCTCCACGCCT	AAGAAGCCATTGCAATTGCC	21	20	42.9	45.0	57.4	57.9	0.0	26.8	1.732679	61566	62325	875-905	1.000000	70.5
accD-psaI_p2	accD-psaI	2	TCCAGAAGGGTTTATTCGACC	AAGAAGCCATTGCAATTGCC	21	20	47.6	45.0	57.4	57.9	0.0	26.8	1.725703	61566	62325	935-965	1.000000	70.3
accD-psaI_p3	accD-psaI	3	CCAGAAGGGTTTATTCGACCT	AAGAAGCCATTGCAATTGCC	21	20	47.6	45.0	57.4	57.9	0.0	26.8	1.732679	61566	62325	934-964	1.000000	70.2
accD-psaI_p4	accD-psaI	4	TCGACCTAATTGTACCACGT	AAGAAGCCATTGCAATTGCC	20	20	45.0	45.0	56.3	57.9	0.0	26.8	1.795426	61566	62325	920-950	1.000000	69.8
accD-psaI_p5	accD-psaI	5	TCCAGAAGGGTTTATTCGACC	TGCAATTGCCGGAAAGACTA	21	20	47.6	45.0	57.4	57.8	0.0	26.9	1.814829	61566	62325	925-955	1.000000	69.6
psaJ-rpl33_p1	psaJ-rpl33	1	ATGCGTTGACATTCCCCTTT	CTCGGACATCTTTCCCCTTG	20	20	45.0	55.0	58.1	58.0	0.0	0.0	0.106483	70336	70771	481-493	1.000000	83.2
psaJ-rpl33_p2	psaJ-rpl33	2	GGCACCAGTACTAAGTACGC	CTCGGACATCTTTCCCCTTG	20	20	55.0	55.0	58.1	58.0	0.0	0.0	0.111244	70336	70771	555-567	1.000000	82.6
psaJ-rpl33_p3	psaJ-rpl33	3	TGCGTTGACATTCCCCTTTT	CTCGGACATCTTTCCCCTTG	20	20	45.0	55.0	58.3	58.0	0.0	0.0	0.331332	70336	70771	480-492	1.000000	81.4
psaJ-rpl33_p4	psaJ-rpl33	4	ATCTCTCTGTGGCACCAGTA	CTCGGACATCTTTCCCCTTG	20	20	50.0	55.0	57.8	58.0	16.3	0.0	0.267922	70336	70771	565-577	1.000000	81.3
psaJ-rpl33_p5	psaJ-rpl33	5	TATCTCTCTGTGGCACCAGT	CTCGGACATCTTTCCCCTTG	20	20	50.0	55.0	57.8	58.0	16.3	0.0	0.267922	70336	70771	566-578	1.000000	81.3
rpl20_p1	rpl20	1	AGATTTGAGTATCGTGTCGT	TCGTCAAAGTTTCATTTATTCA	20	22	40.0	27.3	54.3	52.4	0.0	0.0	11.370398	71641	72105	568-616	1.000000	51.5
rpl20_p2	rpl20	2	AGATTTGAGTATCGTGTCGT	AGTTATTCGTCAAAGTTTCATT	20	22	40.0	27.3	54.3	52.3	0.0	0.0	11.393474	71641	72105	574-622	1.000000	51.5
rpl20_p3	rpl20	3	AGATTTGAGTATCGTGTCGT	TTCGTCAAAGTTTCATTTATTCA	20	23	40.0	26.1	54.3	53.2	0.0	0.0	11.512177	71641	72105	569-617	1.000000	51.5
rpl20_p4	rpl20	4	AGATTTGAGTATCGTGTCGT	AGTTATTCGTCAAAGTTTCATTT	20	23	40.0	26.1	54.3	53.2	0.0	0.0	11.531832	71641	72105	574-622	1.000000	51.5
rpl20_p5	rpl20	5	TCTAGATTTGAGTATCGTGTCGT	TCGTCAAAGTTTCATTTATTCA	23	22	39.1	27.3	56.9	52.4	0.0	0.0	11.694655	71641	72105	571-619	1.000000	51.5
rps19-rpl2_p1	rps19-rpl2	1	GCCCGGGACCAAGTTACTAT	TTGATTCTTCGTCGCCGTAG	20	20	55.0	50.0	59.2	58.1	29.3	0.0	1.260580	87433	87508	199-213	1.000000	75.4
rps19-rpl2_p2	rps19-rpl2	2	GCCCGGGACCAAGTTACTAT	CACAACCCCTTGGGGTTATC	20	20	55.0	55.0	59.2	58.2	29.3	35.0	1.317396	87433	87508	266-280	1.000000	74.6
rps19-rpl2_p3	rps19-rpl2	3	GCCCGGGACCAAGTTACTAT	TTGGGGTTATCCTGCACTTG	20	20	55.0	50.0	59.2	57.8	29.3	0.0	1.379100	87433	87508	257-271	1.000000	74.2
rps19-rpl2_p4	rps19-rpl2	4	GCCCGGGACCAAGTTACTAT	ATTTGATTCTTCGTCGCCGT	20	20	55.0	45.0	59.2	58.3	29.3	0.0	1.442713	87433	87508	201-215	1.000000	74.0
rps19-rpl2_p5	rps19-rpl2	5	CCCGGGACCAAGTTACTATT	TTGATTCTTCGTCGCCGTAG	20	20	50.0	50.0	56.6	58.1	18.5	0.0	1.483856	87433	87508	198-212	1.000000	73.7
ycf1_p1	ycf1	1	ACAGATGTCGTTTCTAGTCT	GCGGACGCATCTCTTCTTAT	20	20	40.0	50.0	53.5	57.9	0.0	12.3	4.676766	112022	113182	1253-1393	1.000000	47.2
ycf1_p2	ycf1	2	AACAGATGTCGTTTCTAGTCT	GCGGACGCATCTCTTCTTAT	21	20	38.1	50.0	54.4	57.9	0.0	12.3	4.785797	112022	113182	1254-1394	1.000000	46.3
ycf1_p3	ycf1	3	AGAAATAAGGGAGGTTTGTG	GCGGACGCATCTCTTCTTAT	20	20	40.0	50.0	52.7	57.9	0.0	12.3	5.394173	112022	113182	1158-1297	1.000000	44.6
ycf1_p4	ycf1	4	ACAGATGTCGTTTCTAGTCTATCTC	GCGGACGCATCTCTTCTTAT	25	20	40.0	50.0	57.7	57.9	0.0	12.3	5.395763	112022	113182	1253-1393	1.000000	44.6
ycf1_p5	ycf1	5	ACAGATGTCGTTTCTAGTCTATCT	GCGGACGCATCTCTTCTTAT	24	20	37.5	50.0	56.7	57.9	0.0	12.3	5.398313	112022	113182	1253-1393	1.000000	44.6
ndhF-rpl32_p1	ndhF-rpl32	1	TGAAAGGTATGATCCACGCA	TGATCTCGAAGTGCGTTTTT	20	20	45.0	40.0	56.9	56.0	0.0	0.0	3.059112	115385	115637	326-863	1.000000	60.8
ndhF-rpl32_p2	ndhF-rpl32	2	TGAAAGGTATGATCCACGCA	TTGATCTCGAAGTGCGTTTT	20	20	45.0	40.0	56.9	56.0	0.0	0.0	3.059112	115385	115637	327-864	1.000000	60.8
ndhF-rpl32_p3	ndhF-rpl32	3	TGAAAGGTATGATCCACGCA	TTTGATCTCGAAGTGCGTTT	20	20	45.0	40.0	56.9	56.0	0.0	0.0	3.059112	115385	115637	328-865	1.000000	60.8
ndhF-rpl32_p4	ndhF-rpl32	4	TGAAAGGTATGATCCACGCA	TTTTGATCTCGAAGTGCGTT	20	20	45.0	40.0	56.9	56.0	0.0	0.0	3.059112	115385	115637	329-866	1.000000	60.8
ndhF-rpl32_p5	ndhF-rpl32	5	TGAAAGGTATGATCCACGCA	TTTTTGATCTCGAAGTGCGT	20	20	45.0	40.0	56.9	56.0	0.0	0.0	3.059112	115385	115637	330-867	1.000000	60.8
rpl32-trnL_p1	rpl32-trnL	1	TCTACTGGGGAGTCAAAAGGT	TCCTAAGAGCAGCGTGTCTA	21	20	47.6	50.0	58.0	57.9	0.0	0.0	1.138227	115809	116678	966-966	0.066667	33.6
rpl32-trnL_p2	rpl32-trnL	2	TCTACTGGGGAGTCAAAAGGT	TCGAACCGAGATGCATTAGC	21	20	47.6	50.0	58.0	58.1	0.0	0.0	1.161008	115809	116678	992-992	0.066667	33.4
rpl32-trnL_p3	rpl32-trnL	3	TCTACTGGGGAGTCAAAAGGT	AGCAGCGTGTCTACCAATTT	21	20	47.6	45.0	58.0	57.8	0.0	0.0	1.198954	115809	116678	959-959	0.066667	33.1
rpl32-trnL_p4	rpl32-trnL	4	TCTACTGGGGAGTCAAAAGGT	CCTAAGAGCAGCGTGTCTAC	21	20	47.6	55.0	58.0	57.8	0.0	0.0	1.210245	115809	116678	965-965	0.066667	33.0
rpl32-trnL_p5	rpl32-trnL	5	TCTACTGGGGAGTCAAAAGGT	CTAAGAGCAGCGTGTCTACC	21	20	47.6	55.0	58.0	57.8	0.0	0.0	1.210245	115809	116678	964-964	0.066667	33.0
ycf1_p1	ycf1	1	GAAATGGTCGATTCCACCGA	TCACCTTACAGAGAGAGGCT	20	20	50.0	50.0	58.0	57.1	0.0	0.0	0.928046	130883	131002	215-233	1.000000	78.7
ycf1_p2	ycf1	2	GAAATGGTCGATTCCACCGA	CTCACCTTACAGAGAGAGGCT	20	21	50.0	52.4	58.0	58.3	0.0	0.0	1.284586	130883	131002	216-234	1.000000	75.9
ycf1_p3	ycf1	3	ACGGCTTGGTCAAATCGATT	TCACCTTACAGAGAGAGGCT	20	20	45.0	50.0	58.2	57.1	4.4	0.0	1.088287	130883	131002	249-267	0.933333	72.4
ycf1_p4	ycf1	4	GAAATGGTCGATTCCACCGA	CTCACCTTACAGAGAGAGGC	20	20	50.0	55.0	58.0	56.8	0.0	0.0	1.264840	130883	131002	216-234	0.933333	71.5
ycf1_p5	ycf1	5	GAAATGGTCGATTCCACCGA	TCTCACCTTACAGAGAGAGGC	20	21	50.0	52.4	58.0	58.3	0.0	0.0	1.289348	130883	131002	217-235	0.933333	71.3