Hotspot

clpP

LSC · 69418-71454 · 2037 bp

Back to Capsella

Pi 0.00295

MarkerSeek score 36.10

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2037
variable_sites: 9
pi: 0.00295
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 36.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	AAATGGAATTTCCCCCGCTT	ATACGCAATGGGGGTGAATC	45.0	50.0	58.0	58.0	2866-2876 bp	1.0	80.5
clpP_p2	AAATGGAATTTCCCCCGCTT	ACCGAAACCCCAATCTTACG	45.0	50.0	58.0	57.9	2641-2657 bp	1.0	79.9
clpP_p3	AAATGGAATTTCCCCCGCTT	CCGAAACCCCAATCTTACGT	45.0	50.0	58.0	57.9	2640-2656 bp	1.0	79.9
clpP_p4	CATTCTTTGGCTCTACCCCC	ACCGAAACCCCAATCTTACG	55.0	50.0	57.9	57.9	2796-2812 bp	1.0	79.7
clpP_p5	CATTCTTTGGCTCTACCCCC	CCGAAACCCCAATCTTACGT	55.0	50.0	57.9	57.9	2795-2811 bp	1.0	79.7