Hotspot

ndhF-rpl32

SSC · 112399-113222 · 824 bp

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Pi 0.0103

MarkerSeek score 64.10

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 824
variable_sites: 13
pi: 0.0103
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	AGATGCGGCGAAGGTTTTT	ACGATGTCCAATATCCCTTCC	47.4	47.6	58.4	57.2	1026-1059 bp	1.0	56.2
ndhF-rpl32_p2	CTGGAAGTGGAATGAAAGGGA	ACGATGTCCAATATCCCTTCC	47.6	47.6	57.6	57.2	960-993 bp	1.0	55.6
ndhF-rpl32_p3	TTGTTGCTGTCGGAAAAAGT	ACGATGTCCAATATCCCTTCC	40.0	47.6	56.4	57.2	1008-1041 bp	1.0	54.3
ndhF-rpl32_p4	AGATGCGGCGAAGGTTTTT	CGATGTCCAATATCCCTTCCT	47.4	47.6	58.4	56.9	1025-1058 bp	1.0	53.9
ndhF-rpl32_p5	TGGAAGTGGAATGAAAGGGA	ACGATGTCCAATATCCCTTCC	45.0	47.6	56.3	57.2	959-992 bp	1.0	53.8