Hotspot

rps12-clpP

LSC · 69238-69417 · 180 bp

Back to Capsella

Pi 0.0296

MarkerSeek score 51.70

Primer available yes

Label rps12-clpP

Feature Metadata

label_name: rps12-clpP
feature_type: igs
region: LSC
length_bp: 180
variable_sites: 8
pi: 0.0296
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 51.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-clpP_p1	CGAGTACATGTTCCTCGTCG	TATGTCAGCAACAGAAGCCC	55.0	50.0	58.2	57.9	344-345 bp	1.0	79.7
rps12-clpP_p2	CCGAGTACATGTTCCTCGTC	TATGTCAGCAACAGAAGCCC	55.0	50.0	57.8	57.9	345-346 bp	1.0	79.7
rps12-clpP_p3	TTTCGGATTGGCTGTCTTGT	TATGTCAGCAACAGAAGCCC	45.0	50.0	57.7	57.9	287-288 bp	1.0	79.4
rps12-clpP_p4	CACCCGAGTACATGTTCCTC	TATGTCAGCAACAGAAGCCC	55.0	50.0	57.7	57.9	348-349 bp	1.0	78.8
rps12-clpP_p5	ACACCCGAGTACATGTTCCT	TATGTCAGCAACAGAAGCCC	50.0	50.0	58.4	57.9	349-350 bp	1.0	78.3