Hotspot

rpl20-rps12

LSC · 68357-69123 · 767 bp

Back to Capsella

Pi 0.00478
MarkerSeek score 62.20
Primer available yes
Label rpl20-rps12

Feature Metadata

label_name
rpl20-rps12
feature_type
igs
region
LSC
length_bp
767
variable_sites
6
pi
0.00478
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl20-rps12_p1 TCCGAGCTATATATCCGCGT ACAAGACAGCCAATCCGAAA 50.0 45.0 58.0 57.7 884-886 bp 1.0 78.3
rpl20-rps12_p2 TCCGAGCTATATATCCGCGT GAGGAACATGTACTCGGGTG 50.0 55.0 58.0 57.7 823-825 bp 1.0 78.1
rpl20-rps12_p3 TTTTGTTCTACGCCTCCGAG ACAAGACAGCCAATCCGAAA 50.0 45.0 57.9 57.7 898-900 bp 1.0 78.0
rpl20-rps12_p4 TTTTGTTCTACGCCTCCGAG GAGGAACATGTACTCGGGTG 50.0 55.0 57.9 57.7 837-839 bp 1.0 77.8
rpl20-rps12_p5 TCCGAGCTATATATCCGCGT AGGAACATGTACTCGGGTGT 50.0 50.0 58.0 58.4 822-824 bp 1.0 77.6