Hotspot

petA-psbJ

LSC · 62122-63099 · 978 bp

Back to Capsella

Pi 0.00801

MarkerSeek score 54.50

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 978
variable_sites: 12
pi: 0.00801
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ACGTGTCCAAGGTCTTTTGT	GCTGGTATTCCTGTGATCGG	45.0	55.0	57.6	58.1	1165-1175 bp	1.0	76.5
petA-psbJ_p2	TAGGATCGGTTGTTTTGGCA	GCTGGTATTCCTGTGATCGG	45.0	55.0	57.4	58.1	1139-1149 bp	1.0	75.2
petA-psbJ_p3	TCCATTACGTGTCCAAGGTC	GCTGGTATTCCTGTGATCGG	50.0	55.0	57.2	58.1	1171-1181 bp	1.0	73.7
petA-psbJ_p4	CCATTACGTGTCCAAGGTCT	GCTGGTATTCCTGTGATCGG	50.0	55.0	57.2	58.1	1170-1180 bp	1.0	73.7
petA-psbJ_p5	AGGATCGGTTGTTTTGGCAC	GCTGGTATTCCTGTGATCGG	50.0	55.0	59.0	58.1	1138-1148 bp	1.0	71.5