Hotspot

rbcL-accD

LSC · 55933-56644 · 712 bp

Back to Capsella

Pi 0.0103

MarkerSeek score 54.90

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 712
variable_sites: 12
pi: 0.0103
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 54.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	CGTGAAGCTTGCAAATGGAG	GCTTTCACGAATCTTGCCAC	50.0	50.0	58.0	58.0	1370-1399 bp	1.0	82.2
rbcL-accD_p2	AATGAGGGACGTGATCTTGC	GCTTTCACGAATCTTGCCAC	50.0	50.0	58.0	58.0	1412-1441 bp	1.0	82.1
rbcL-accD_p3	TGCAATGCACGCTGTTATTG	GCTTTCACGAATCTTGCCAC	45.0	50.0	58.0	58.0	1821-1850 bp	1.0	81.6
rbcL-accD_p4	TCATGGTATGCACTTCCGTG	GCTTTCACGAATCTTGCCAC	50.0	50.0	58.0	58.0	1788-1817 bp	1.0	81.6
rbcL-accD_p5	TTGAATGCTACTGCGGGTAC	GCTTTCACGAATCTTGCCAC	50.0	50.0	58.0	58.0	1988-2017 bp	1.0	81.5