Hotspot

ndhC-trnV

LSC · 49895-50733 · 839 bp

Back to Capsella

Pi 0.0114

MarkerSeek score 53.30

Primer available yes

Label ndhC-trnV

Feature Metadata

label_name: ndhC-trnV
feature_type: igs
region: LSC
length_bp: 839
variable_sites: 15
pi: 0.0114
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV_p1	CTCCGGCCCTTTCCTAATTG	GTTCGAGTCCGTATAGCCCT	55.0	55.0	58.3	58.7	977-986 bp	1.0	73.6
ndhC-trnV_p2	TCTCCGGCCCTTTCCTAATT	GTTCGAGTCCGTATAGCCCT	50.0	55.0	58.4	58.7	978-987 bp	1.0	72.9
ndhC-trnV_p3	TTCTCCGGCCCTTTCCTAAT	GTTCGAGTCCGTATAGCCCT	50.0	55.0	58.4	58.7	979-988 bp	1.0	72.9
ndhC-trnV_p4	CTCCGGCCCTTTCCTAATTG	TTCGAGTCCGTATAGCCCTA	55.0	50.0	58.3	57.1	976-985 bp	1.0	71.6
ndhC-trnV_p5	TTTCTCCGGCCCTTTCCTAA	GTTCGAGTCCGTATAGCCCT	50.0	55.0	58.6	58.7	980-989 bp	1.0	71.1