Hotspot

rpoB-trnC

LSC · 26155-27252 · 1098 bp

Back to Capsella

Pi 0.00531

MarkerSeek score 52.90

Primer available yes

Label rpoB-trnC

Feature Metadata

label_name: rpoB-trnC
feature_type: igs
region: LSC
length_bp: 1098
variable_sites: 9
pi: 0.00531
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC_p1	CCGGGTATTGTAGATGTTCCC	CCGGATTTGAACTGGGGAAA	52.4	50.0	57.9	58.1	1200-1201 bp	1.0	71.0
rpoB-trnC_p2	GTTCCCTCTTTTTCATCCCCA	CCGGATTTGAACTGGGGAAA	47.6	50.0	57.8	58.1	1185-1186 bp	1.0	70.7
rpoB-trnC_p3	TGTTCCCTCTTTTTCATCCCC	CCGGATTTGAACTGGGGAAA	47.6	50.0	57.8	58.1	1186-1187 bp	1.0	70.7
rpoB-trnC_p4	CCGGGTATTGTAGATGTTCCC	TAAGTCTGTTGTTGAGGCGG	52.4	50.0	57.9	57.8	1223-1224 bp	1.0	70.4
rpoB-trnC_p5	GTTCCCTCTTTTTCATCCCCA	TAAGTCTGTTGTTGAGGCGG	47.6	50.0	57.8	57.8	1208-1209 bp	1.0	70.0