Hotspot

trnG-trnR

LSC · 9241-9397 · 157 bp

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Pi 0.0244

MarkerSeek score 59.90

Primer available yes

Label trnG-trnR

Feature Metadata

label_name: trnG-trnR
feature_type: igs
region: LSC
length_bp: 157
variable_sites: 6
pi: 0.0244
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-trnR_p1	AGCCTTCCAAGCTAACGATG	AGGTTTAGAAGACCTATGTCCT	50.0	40.9	58.0	55.6	241-243 bp	1.0	47.4
trnG-trnR_p2	ACCCTTAGCCTTCCAAGCTA	AGGTTTAGAAGACCTATGTCCT	50.0	40.9	58.0	55.6	247-249 bp	1.0	47.3
trnG-trnR_p3	AGCCTTCCAAGCTAACGATG	AGAAGACCTATGTCCTATCCA	50.0	42.9	58.0	54.6	235-237 bp	1.0	47.2
trnG-trnR_p4	ACCCTTAGCCTTCCAAGCTA	AGAAGACCTATGTCCTATCCA	50.0	42.9	58.0	54.6	241-243 bp	1.0	47.1
trnG-trnR_p5	CGACGTCGACTAAAACCCTT	AGGTTTAGAAGACCTATGTCCT	50.0	40.9	57.9	55.6	261-263 bp	1.0	47.0