Hotspot

rps16-trnQ

LSC · 6147-6541 · 395 bp

Back to Capsella

Pi 0.0152

MarkerSeek score 34.10

Primer available yes

Label rps16-trnQ

Feature Metadata

label_name: rps16-trnQ
feature_type: igs
region: LSC
length_bp: 395
variable_sites: 9
pi: 0.0152
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 34.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ_p1	CGTTGCTTTCTACCACATCG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.2	58.1	460-470 bp	1.0	74.8
rps16-trnQ_p2	GCACGTTGCTTTCTACCACA	GAGGTTCGAATCCTTCCGTC	50.0	55.0	59.1	58.1	463-473 bp	1.0	72.6
rps16-trnQ_p3	CGTTGCTTTCTACCACATCG	ATAAAAAGCGAAATGGGGCG	50.0	45.0	57.2	57.4	521-531 bp	1.0	70.6
rps16-trnQ_p4	CGTTGCTTTCTACCACATCG	TTCGGAGGTTCGAATCCTTC	50.0	50.0	57.2	57.3	464-474 bp	1.0	70.1
rps16-trnQ_p5	CGTTGCTTTCTACCACATCG	TAAGGCAACGGGTTTTGGTC	50.0	50.0	57.2	58.7	490-500 bp	1.0	69.9