Hotspot

trnK-rps16

LSC · 4331-5005 · 675 bp

Back to Capsella

Pi 0.0126

MarkerSeek score 53.50

Primer available yes

Label trnK-rps16

Feature Metadata

label_name: trnK-rps16
feature_type: igs
region: LSC
length_bp: 675
variable_sites: 14
pi: 0.0126
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 53.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-rps16_p1	CTACCGCAGATTTGGACGAA	AAAGGAGCTCACCCAACAAG	50.0	50.0	58.0	58.0	860-866 bp	1.0	82.0
trnK-rps16_p2	CTACCGCAGATTTGGACGAA	AAGGATTCTCCGGCGAGATA	50.0	50.0	58.0	58.0	1255-1260 bp	1.0	81.5
trnK-rps16_p3	CTACCGCAGATTTGGACGAA	TAAGGATTCTCCGGCGAGAT	50.0	50.0	58.0	58.0	1256-1261 bp	1.0	81.5
trnK-rps16_p4	CCTTTCAGGATCAGTCGTGG	AAGGATTCTCCGGCGAGATA	55.0	50.0	58.0	58.0	1286-1291 bp	1.0	81.4
trnK-rps16_p5	CCTTTCAGGATCAGTCGTGG	TAAGGATTCTCCGGCGAGAT	55.0	50.0	58.0	58.0	1287-1292 bp	1.0	81.4