Hotspot

trnH-psbA

LSC · 84-373 · 290 bp

Back to Capsella

Pi 0.0224

MarkerSeek score 67.30

Primer available yes

Label trnH-psbA

Feature Metadata

label_name: trnH-psbA
feature_type: igs
region: LSC
length_bp: 290
variable_sites: 11
pi: 0.0224
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-psbA_p1	CTGCCTTAATCCACTTGGCT	CCTCTAGACCTAGCTGCTGT	50.0	55.0	57.9	57.9	363-365 bp	1.0	80.8
trnH-psbA_p2	CTGCCTTAATCCACTTGGCT	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.9	57.9	366-368 bp	1.0	80.7
trnH-psbA_p3	ACTGCCTTAATCCACTTGGC	CCTCTAGACCTAGCTGCTGT	50.0	55.0	58.2	57.9	364-366 bp	1.0	80.6
trnH-psbA_p4	ACTGCCTTAATCCACTTGGC	TTCCCTCTAGACCTAGCTGC	50.0	55.0	58.2	57.9	367-369 bp	1.0	80.5
trnH-psbA_p5	AATCCACTTGGCTACATCCG	CCTCTAGACCTAGCTGCTGT	50.0	55.0	57.7	57.9	356-358 bp	1.0	79.3