Hotspot

ycf1

SSC · 126872-132382 · 5511 bp

Back to Capparis

Pi 0.0156

MarkerSeek score 55.70

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5511
variable_sites: 229
pi: 0.0156
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TAATAAGTTGTCCATCGTTTTT	TGGTTAGACTGATAAATCCAAACG	27.3	37.5	52.1	57.0	174 bp	1.0	48.0
ycf1_p2	ATAATAAGTTGTCCATCGTTTTT	TGGTTAGACTGATAAATCCAAACG	26.1	37.5	52.7	57.0	175 bp	1.0	48.0
ycf1_p3	TAATAAGTTGTCCATCGTTTTT	TTGGTTAGACTGATAAATCCAAACG	27.3	36.0	52.1	57.6	175 bp	1.0	48.0
ycf1_p4	TAATAAGTTGTCCATCGTTTTT	GGTTAGACTGATAAATCCAAACG	27.3	39.1	52.1	55.4	173 bp	1.0	48.0
ycf1_p5	ATAATAAGTTGTCCATCGTTTTT	TTGGTTAGACTGATAAATCCAAACG	26.1	36.0	52.7	57.6	176 bp	1.0	48.0