Hotspot

psbF-psbE

LSC · 66243-66251 · 9 bp

Back to Capparis

Pi 0.037

MarkerSeek score 61.40

Primer available yes

Label psbF-psbE

Feature Metadata

label_name: psbF-psbE
feature_type: igs
region: LSC
length_bp: 9
variable_sites: 1
pi: 0.037
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbF-psbE_p1	GCACAGCTAGTCCATGAACA	AACTGGCCGTTTTGATCCTT	50.0	45.0	57.9	58.0	131 bp	1.0	79.8
psbF-psbE_p2	GCACAGCTAGTCCATGAACA	ACTGGCCGTTTTGATCCTTT	50.0	45.0	57.9	58.0	130 bp	1.0	79.8
psbF-psbE_p3	GCTAGTCCATGAACAGCCAA	AACTGGCCGTTTTGATCCTT	50.0	45.0	57.9	58.0	126 bp	1.0	79.7
psbF-psbE_p4	GCTAGTCCATGAACAGCCAA	ACTGGCCGTTTTGATCCTTT	50.0	45.0	57.9	58.0	125 bp	1.0	79.7
psbF-psbE_p5	TAGGCACAGCTAGTCCATGA	ACTGGCCGTTTTGATCCTTT	50.0	45.0	57.8	58.0	133 bp	1.0	79.3