Hotspot

rbcL-accD

LSC · 58428-59112 · 685 bp

Back to Capparis

Pi 0.0159
MarkerSeek score 61.70
Primer available yes
Label rbcL-accD

Feature Metadata

label_name
rbcL-accD
feature_type
igs
region
LSC
length_bp
685
variable_sites
28
pi
0.0159
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.99
markerseek_score
61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rbcL-accD_p1 AGATCTCCCGGCTACTAAGT TCAAATTGAACCACGATTTTTCCA 50.0 33.3 56.9 58.1 725-732 bp 1.0 45.6
rbcL-accD_p2 AGATCTCCCGGCTACTAAGT CAAATTGAACCACGATTTTTCCA 50.0 34.8 56.9 56.8 724-731 bp 1.0 45.6
rbcL-accD_p3 AGATCTCCCGGCTACTAAGT ATTGAACCACGATTTTTCCA 50.0 35.0 56.9 53.7 721-728 bp 1.0 45.6
rbcL-accD_p4 AGATCTCCCGGCTACTAAGT TTGAACCACGATTTTTCCAT 50.0 35.0 56.9 53.7 720-727 bp 1.0 45.6
rbcL-accD_p5 AGATCTCCCGGCTACTAAGT AATTGAACCACGATTTTTCCA 50.0 33.3 56.9 54.6 722-729 bp 1.0 45.6