Hotspot

trnF-GAA-ndhJ

LSC · 49880-50476 · 597 bp

Back to Capparis

Pi 0.0233

MarkerSeek score 63.30

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 597
variable_sites: 30
pi: 0.0233
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	CCCGAAAGTTGGATAGGGTG	50.0	55.0	58.1	58.0	697-709 bp	1.0	85.2
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	CCGAAAGTTGGATAGGGTGG	50.0	55.0	58.1	58.0	696-708 bp	1.0	85.2
trnF-GAA-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	ACCATCCACGACTAAAACGG	50.0	50.0	58.1	57.9	726-738 bp	1.0	84.5
trnF-GAA-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	GAAAGTTGGATAGGGTGGCC	50.0	55.0	58.1	58.2	694-706 bp	1.0	83.6
trnF-GAA-ndhJ_p5	CCTCGTGTCACCAGTTCAAA	ATGCCCGAAAGTTGGATAGG	50.0	50.0	58.1	57.7	700-712 bp	1.0	82.7