Hotspot

trnS-UGA-psbZ

LSC · 37168-37518 · 351 bp

Back to Capparis

Pi 0.0279

MarkerSeek score 60.50

Primer available yes

Label trnS-UGA-psbZ

Feature Metadata

label_name: trnS-UGA-psbZ
feature_type: igs
region: LSC
length_bp: 351
variable_sites: 24
pi: 0.0279
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-UGA-psbZ_p1	TTTTCAAGACCGGAGCCATC	CCAACCATCAGGAGAAGCAA	50.0	50.0	58.2	57.8	490-501 bp	1.0	80.8
trnS-UGA-psbZ_p2	TTTTCAAGACCGGAGCCATC	AACCAACCATCAGGAGAAGC	50.0	50.0	58.2	57.8	492-503 bp	1.0	80.8
trnS-UGA-psbZ_p3	GTTTTCAAGACCGGAGCCAT	CCAACCATCAGGAGAAGCAA	50.0	50.0	58.5	57.8	491-502 bp	1.0	78.5
trnS-UGA-psbZ_p4	GTTTTCAAGACCGGAGCCAT	AACCAACCATCAGGAGAAGC	50.0	50.0	58.5	57.8	493-504 bp	1.0	78.5
trnS-UGA-psbZ_p5	TTTTCAAGACCGGAGCCATC	ACTCAACCAACCATCAGGAG	50.0	50.0	58.2	57.1	496-507 bp	1.0	75.5