Hotspot

trnR-UCU-atpA

LSC · 10138-10419 · 282 bp

Back to Capparis

Pi 0.0312

MarkerSeek score 64.60

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 282
variable_sites: 18
pi: 0.0312
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	AGTGAAAAGCGTCCATTGTCT	CTACCAAGGCATTCACCGAA	42.9	50.0	58.1	57.9	445-494 bp	1.0	77.1
trnR-UCU-atpA_p2	AGTGAAAAGCGTCCATTGTCT	TACCAAGGCATTCACCGAAG	42.9	50.0	58.1	57.9	444-493 bp	1.0	77.1
trnR-UCU-atpA_p3	AGTGAAAAGCGTCCATTGTCT	TTCTACCAAGGCATTCACCG	42.9	50.0	58.1	57.9	447-496 bp	1.0	77.1
trnR-UCU-atpA_p4	GTGAAAAGCGTCCATTGTCT	CTACCAAGGCATTCACCGAA	45.0	50.0	56.6	57.9	444-493 bp	1.0	75.5
trnR-UCU-atpA_p5	GTGAAAAGCGTCCATTGTCT	TACCAAGGCATTCACCGAAG	45.0	50.0	56.6	57.9	443-492 bp	1.0	75.5