Hotspot

ycf3

LSC · 43187-45200 · 2014 bp

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Pi 0

MarkerSeek score 57.70

Primer available yes

Label ycf3

Feature Metadata

label_name: ycf3
feature_type: gene
region: LSC
length_bp: 2014
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3_p1	ACACATACAAAGAGACCCGC	AATGCCCTTGTTTGTGGGAT	50.0	45.0	57.9	58.0	2737-2738 bp	1.0	79.0
ycf3_p2	ACACATACAAAGAGACCCGC	ATGCCCTTGTTTGTGGGATT	50.0	45.0	57.9	58.0	2736-2737 bp	1.0	79.0
ycf3_p3	CATACAAAGAGACCCGCCAA	AATGCCCTTGTTTGTGGGAT	50.0	45.0	57.9	58.0	2734-2735 bp	1.0	78.9
ycf3_p4	CATACAAAGAGACCCGCCAA	ATGCCCTTGTTTGTGGGATT	50.0	45.0	57.9	58.0	2733-2734 bp	1.0	78.9
ycf3_p5	ACACATACAAAGAGACCCGC	ACAAAACGGGCGGTTATTCT	50.0	45.0	57.9	58.1	2886-2887 bp	1.0	78.5