Hotspot

trnR-ACG

SSC · 129917-129990 · 74 bp

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Pi 0.0135

MarkerSeek score 60.30

Primer available yes

Label trnR-ACG

Feature Metadata

label_name: trnR-ACG
feature_type: tRNA
region: SSC
length_bp: 74
variable_sites: 1
pi: 0.0135
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-ACG_p1	ACGCCCAAATTCCATAGCTT	AGGCGCTTTCACATCTTCTT	45.0	45.0	57.9	57.8	260 bp	1.0	77.6
trnR-ACG_p2	AAGGCCATTCCATTTCGACA	AGGCGCTTTCACATCTTCTT	45.0	45.0	57.8	57.8	288 bp	1.0	77.0
trnR-ACG_p3	AGGCCATTCCATTTCGACAA	AGGCGCTTTCACATCTTCTT	45.0	45.0	57.8	57.8	287 bp	1.0	77.0
trnR-ACG_p4	ACGCCCAAATTCCATAGCTT	AGAGGCGCTTTCACATCTTC	45.0	50.0	57.9	58.3	262 bp	1.0	77.0
trnR-ACG_p5	ACGCCCAAATTCCATAGCTT	GAGGCGCTTTCACATCTTCT	45.0	50.0	57.9	58.3	261 bp	1.0	77.0