Hotspot

rps16

LSC · 5271-6421 · 1151 bp

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Pi 0.00261

MarkerSeek score 60.20

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1151
variable_sites: 3
pi: 0.00261
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	AGCAGAGAAAAGATTATAATCCGA	AGTACTAACCGAACCAATGA	33.3	40.0	55.8	53.7	1271-1272 bp	1.0	40.2
rps16_p2	AGCAGAGAAAAGATTATAATCCGA	AAGTACTAACCGAACCAATGA	33.3	38.1	55.8	54.6	1272-1273 bp	1.0	40.2
rps16_p3	AGCAGAGAAAAGATTATAATCCGAAC	AGTACTAACCGAACCAATGA	34.6	40.0	57.6	53.7	1271-1272 bp	1.0	40.2
rps16_p4	GCAGAGAAAAGATTATAATCCGAACT	AGTACTAACCGAACCAATGA	34.6	40.0	57.6	53.7	1270-1271 bp	1.0	40.2
rps16_p5	GCAGAGAAAAGATTATAATCCGAACT	AAGTACTAACCGAACCAATGA	34.6	38.1	57.6	54.6	1271-1272 bp	1.0	40.2