Hotspot

psaB

LSC · 38033-40237 · 2205 bp

Back to Cannabis

Pi 0.000454

MarkerSeek score 57.50

Primer available yes

Label psaB

Feature Metadata

label_name: psaB
feature_type: gene
region: LSC
length_bp: 2205
variable_sites: 1
pi: 0.000454
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaB_p1	GCCTCTTCTTCTCCCTCTGA	AAGGACGTGCTGTAGGAGTA	55.0	50.0	58.1	57.8	2408 bp	1.0	77.2
psaB_p2	GCCTCTTCTTCTCCCTCTGA	AGGACGTGCTGTAGGAGTAA	55.0	50.0	58.1	57.8	2407 bp	1.0	77.2
psaB_p3	GCCTCTTCTTCTCCCTCTGA	GACGTGCTGTAGGAGTAACC	55.0	55.0	58.1	57.7	2405 bp	1.0	76.7
psaB_p4	GCCTCTTCTTCTCCCTCTGA	GGACGTGCTGTAGGAGTAAC	55.0	55.0	58.1	57.7	2406 bp	1.0	76.7
psaB_p5	TTGCCTCTTCTTCTCCCTCT	AGGACGTGCTGTAGGAGTAA	50.0	50.0	57.7	57.8	2409 bp	1.0	75.7