Hotspot

ndhD

SSC · 115005-116507 · 1503 bp

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Pi 0.000665

MarkerSeek score 59.10

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1503
variable_sites: 1
pi: 0.000665
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	AGTGTACAAAACCAACAAACT	CAAAAACCCGCGCTCAAAAT	33.3	45.0	54.1	58.5	1633-1636 bp	1.0	40.0
ndhD_p2	AGTGTACAAAACCAACAAACT	TTTATCGACAAAAACCCGCG	33.3	45.0	54.1	57.4	1641-1644 bp	1.0	40.0
ndhD_p3	AAGTGTACAAAACCAACAAACT	CAAAAACCCGCGCTCAAAAT	31.8	45.0	54.9	58.5	1634-1637 bp	1.0	40.0
ndhD_p4	AGTGTACAAAACCAACAAACTT	CAAAAACCCGCGCTCAAAAT	31.8	45.0	54.9	58.5	1633-1636 bp	1.0	40.0
ndhD_p5	AGTGTACAAAACCAACAAACTT	TTTATCGACAAAAACCCGCG	31.8	45.0	54.9	57.4	1641-1644 bp	1.0	40.0