Hotspot

matK

LSC · 2044-3573 · 1530 bp

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Pi 0.000656

MarkerSeek score 59.40

Primer available yes

Label matK

Feature Metadata

label_name: matK
feature_type: gene
region: LSC
length_bp: 1530
variable_sites: 1
pi: 0.000656
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK_p1	TTCCACGGTCGAATAACCAA	ACCTTGTTTGGACTGTATCGT	45.0	42.9	57.2	57.2	1629-1635 bp	1.0	59.5
matK_p2	TTTCCACGGTCGAATAACCA	ACCTTGTTTGGACTGTATCGT	45.0	42.9	57.2	57.2	1630-1636 bp	1.0	59.5
matK_p3	TTTCCACGGTCGAATAACCAA	ACCTTGTTTGGACTGTATCGT	42.9	42.9	57.9	57.2	1630-1636 bp	1.0	57.2
matK_p4	ATTTCCACGGTCGAATAACCA	ACCTTGTTTGGACTGTATCGT	42.9	42.9	57.7	57.2	1631-1637 bp	1.0	55.5
matK_p5	TTCCACGGTCGAATAACCAA	CCTTGTTTGGACTGTATCGT	45.0	45.0	57.2	55.4	1628-1634 bp	1.0	52.8