Hotspot

accD

LSC · 57247-58740 · 1494 bp

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Pi 0

MarkerSeek score 33.00

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1494
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 33.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	AGCAAAACGATACTACGGGT	TCGTGTTTTGACTTTGATTCCT	45.0	36.4	57.0	56.5	1711-1712 bp	1.0	43.6
accD_p2	AGCAAAACGATACTACGGGTT	TCGTGTTTTGACTTTGATTCCT	42.9	36.4	57.7	56.5	1711-1712 bp	1.0	41.3
accD_p3	AGCAAAACGATACTACGGGT	TTCGTGTTTTGACTTTGATTCCT	45.0	34.8	57.0	57.2	1712-1713 bp	1.0	41.0
accD_p4	AGCAAAACGATACTACGGGT	AGCGAAATCACTATTTTTCACCA	45.0	34.8	57.0	57.0	1680-1681 bp	1.0	40.0
accD_p5	AGCAAAACGATACTACGGGT	TCGTGTTTTGACTTTGATTCC	45.0	38.1	57.0	55.0	1711-1712 bp	1.0	40.0