Hotspot

rpl32-trnL-UAG

SSC · 112827-113630 · 804 bp

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Pi 0.00871
MarkerSeek score 63.80
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
804
variable_sites
7
pi
0.00871
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 AGAAAGGATATTGGGCAGCA AGCGTGTCTACCGATTTCAC 45.0 50.0 56.9 58.0 928-936 bp 1.0 71.5
rpl32-trnL-UAG_p2 AGAAAGGATATTGGGCAGCA CAGCGTGTCTACCGATTTCA 45.0 50.0 56.9 58.0 929-937 bp 1.0 71.5
rpl32-trnL-UAG_p3 AGAAAGGATATTGGGCAGCA TCCTAAGAGCAGCGTGTCTA 45.0 50.0 56.9 57.9 938-946 bp 1.0 70.6
rpl32-trnL-UAG_p4 AGAAAGGATATTGGGCAGCA CCTAAGAGCAGCGTGTCTAC 45.0 55.0 56.9 57.8 937-945 bp 1.0 70.0
rpl32-trnL-UAG_p5 AGAAAGGATATTGGGCAGCA CTAAGAGCAGCGTGTCTACC 45.0 55.0 56.9 57.8 936-944 bp 1.0 70.0