Hotspot

ndhF-rpl32

SSC · 112295-112667 · 373 bp

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Pi 0

MarkerSeek score 58.80

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 373
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 58.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CCACATACGGCGCAAATTTT	TGCTGCCCAATATCCTTTCT	45.0	45.0	58.0	56.9	557-577 bp	1.0	73.7
ndhF-rpl32_p2	CCACATACGGCGCAAATTTT	TGCTGCCCAATATCCTTTCTT	45.0	42.9	58.0	57.6	557-577 bp	1.0	71.6
ndhF-rpl32_p3	CCACATACGGCGCAAATTTT	AAAGCTTTTAATGCTGCCCA	45.0	40.0	58.0	56.5	568-588 bp	1.0	70.5
ndhF-rpl32_p4	CCACATACGGCGCAAATTTT	AAGCTTTTAATGCTGCCCAA	45.0	40.0	58.0	56.5	567-587 bp	1.0	70.5
ndhF-rpl32_p5	CCACATACGGCGCAAATTTT	ATGCTGCCCAATATCCTTTCT	45.0	42.9	58.0	57.4	558-578 bp	1.0	69.9