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rpl16-rps3

LSC · 81736-82876 · 1141 bp

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Pi 0.000877

MarkerSeek score 59.30

Primer available yes

Label rpl16-rps3

Feature Metadata

label_name: rpl16-rps3
feature_type: igs
region: LSC
length_bp: 1141
variable_sites: 1
pi: 0.000877
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16-rps3_p1	TCGCCCTGCTTCTATTTGTC	CCCTACAAACCATTCGAGCT	50.0	50.0	58.0	57.6	1349-1350 bp	1.0	76.7
rpl16-rps3_p2	CGCCCTGCTTCTATTTGTCT	CCCTACAAACCATTCGAGCT	50.0	50.0	58.0	57.6	1348-1349 bp	1.0	76.7
rpl16-rps3_p3	CTAGATGTGATCCAAGCGGG	CCCTACAAACCATTCGAGCT	55.0	50.0	57.8	57.6	1330-1331 bp	1.0	75.6
rpl16-rps3_p4	TCGCCCTGCTTCTATTTGTC	CCCCTACAAACCATTCGAGC	50.0	55.0	58.0	58.6	1350-1351 bp	1.0	75.0
rpl16-rps3_p5	CGCCCTGCTTCTATTTGTCT	CCCCTACAAACCATTCGAGC	50.0	55.0	58.0	58.6	1349-1350 bp	1.0	74.9