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psbE-petL

LSC · 64900-66018 · 1119 bp

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Pi 0.000894

MarkerSeek score 59.30

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1119
variable_sites: 1
pi: 0.000894
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	AGCTGCTAGTAGAAAACCGA	50.0	45.0	57.9	56.3	1265 bp	1.0	66.0
psbE-petL_p2	AGAACGTTCTCCTGTGCTTC	AGCTGCTAGTAGAAAACCGA	50.0	45.0	57.8	56.3	1188 bp	1.0	65.1
psbE-petL_p3	ACGAATAACCAACCCGCAAT	AGCTGCTAGTAGAAAACCGA	45.0	45.0	58.2	56.3	1274 bp	1.0	65.0
psbE-petL_p4	TGCTGACGAATAACCAACCC	AGCTGCTAGTAGAAAACCGA	50.0	45.0	58.2	56.3	1279 bp	1.0	64.9
psbE-petL_p5	CGAATAACCAACCCGCAATG	AGCTGCTAGTAGAAAACCGA	50.0	45.0	57.8	56.3	1273 bp	1.0	64.9