Hotspot

accD-psaI

LSC · 58741-59548 · 808 bp

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Pi 0.00248

MarkerSeek score 60.20

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 808
variable_sites: 2
pi: 0.00248
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	GGCGTTCTGGGTGAGTTATT	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.9	58.0	947 bp	1.0	79.1
accD-psaI_p2	GGCGTTCTGGGTGAGTTATT	TTGCCGGAAATACTAAGCCC	50.0	50.0	57.9	58.0	938 bp	1.0	78.9
accD-psaI_p3	GGCGTTCTGGGTGAGTTATT	AGAAACCATTGCAATTGCCG	50.0	45.0	57.9	57.9	952 bp	1.0	78.6
accD-psaI_p4	TAAAAGGCGTTCTGGGTGAG	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.8	58.0	952 bp	1.0	78.5
accD-psaI_p5	TAAAAGGCGTTCTGGGTGAG	TTGCCGGAAATACTAAGCCC	50.0	50.0	57.8	58.0	943 bp	1.0	78.4