Hotspot

matK-trnK-UUU

LSC · 3574-4335 · 762 bp

Back to Cannabis

Pi 0

MarkerSeek score 58.90

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 762
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	GGAGGTCATGTTGCCAAGAT	TCGGGTTGCTAACTCAATGG	50.0	50.0	57.9	57.9	856 bp	1.0	78.2
matK-trnK-UUU_p2	GGAGGTCATGTTGCCAAGAT	CGGGTTGCTAACTCAATGGT	50.0	50.0	57.9	58.2	855 bp	1.0	77.6
matK-trnK-UUU_p3	TAGGAGGTCATGTTGCCAAG	TCGGGTTGCTAACTCAATGG	50.0	50.0	57.2	57.9	858 bp	1.0	72.9
matK-trnK-UUU_p4	TAGGAGGTCATGTTGCCAAG	CGGGTTGCTAACTCAATGGT	50.0	50.0	57.2	58.2	857 bp	1.0	72.4
matK-trnK-UUU_p5	AGGAGGTCATGTTGCCAAGA	TCGGGTTGCTAACTCAATGG	50.0	50.0	58.9	57.9	857 bp	1.0	71.8