Hotspot

trnH-GUG-psbA

LSC · 80-400 · 321 bp

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Pi 0.00312
MarkerSeek score 54.70
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
321
variable_sites
1
pi
0.00312
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
54.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG CCCTCTAGACTTAGCTGCTCT 55.0 52.4 58.1 58.1 389 bp 1.0 70.7
trnH-GUG-psbA_p2 CAATCCACTGCCTTGATCCA CCCTCTAGACTTAGCTGCTCT 50.0 52.4 57.9 58.1 403 bp 1.0 70.7
trnH-GUG-psbA_p3 GATCCACTTGGCTACATCCG TCCCTCTAGACTTAGCTGCTC 55.0 52.4 58.1 58.1 390 bp 1.0 70.7
trnH-GUG-psbA_p4 AATCCACTGCCTTGATCCAC CCCTCTAGACTTAGCTGCTCT 50.0 52.4 57.9 58.1 402 bp 1.0 70.7
trnH-GUG-psbA_p5 ACAATCCACTGCCTTGATCC CCCTCTAGACTTAGCTGCTCT 50.0 52.4 57.9 58.1 404 bp 1.0 70.7