primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	GATCCACTTGGCTACATCCG	CCCTCTAGACTTAGCTGCTCT	20	21	55.0	52.4	58.1	58.1	0.0	4.9	1.184790	80	400	389-389	1.000000	70.7
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	CAATCCACTGCCTTGATCCA	CCCTCTAGACTTAGCTGCTCT	20	21	50.0	52.4	57.9	58.1	0.0	4.9	1.189152	80	400	403-403	1.000000	70.7
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	GATCCACTTGGCTACATCCG	TCCCTCTAGACTTAGCTGCTC	20	21	55.0	52.4	58.1	58.1	0.0	4.9	1.190082	80	400	390-390	1.000000	70.7
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	AATCCACTGCCTTGATCCAC	CCCTCTAGACTTAGCTGCTCT	20	21	50.0	52.4	57.9	58.1	0.0	4.9	1.190659	80	400	402-402	1.000000	70.7
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	ACAATCCACTGCCTTGATCC	CCCTCTAGACTTAGCTGCTCT	20	21	50.0	52.4	57.9	58.1	0.0	4.9	1.190659	80	400	404-404	1.000000	70.7
trnK-UUU_p1	trnK-UUU	1	AATGAAATGAGTTAACAAGTTCA	TTTGTTAGGATTCCGATTGT	23	20	26.1	35.0	52.8	52.5	19.7	0.0	13.687037	1727	4372	2747-2753	1.000000	40.2
trnK-UUU_p2	trnK-UUU	2	AATGAAATGAGTTAACAAGTTCA	TGTTAGGATTCCGATTGTATCT	23	22	26.1	36.4	52.8	54.4	19.7	0.0	13.730816	1727	4372	2745-2751	1.000000	40.2
trnK-UUU_p3	trnK-UUU	3	AATGAAATGAGTTAACAAGTTCA	TTTTGTTAGGATTCCGATTGT	23	21	26.1	33.3	52.8	53.4	19.7	0.0	13.743025	1727	4372	2748-2754	1.000000	40.2
trnK-UUU_p4	trnK-UUU	4	AAATGAAATGAGTTAACAAGTTCA	TTTGTTAGGATTCCGATTGT	24	20	25.0	35.0	53.7	52.5	19.7	0.0	13.875455	1727	4372	2748-2754	1.000000	40.2
trnK-UUU_p5	trnK-UUU	5	AATGAAATGAGTTAACAAGTTCA	TTTTTGTTAGGATTCCGATTGT	23	22	26.1	31.8	52.8	54.3	19.7	0.0	13.884628	1727	4372	2749-2755	1.000000	40.2
matK_p1	matK	1	TTCCACGGTCGAATAACCAA	ACCTTGTTTGGACTGTATCGT	20	21	45.0	42.9	57.2	57.2	0.0	0.0	2.599817	2044	3573	1629-1635	1.000000	59.5
matK_p2	matK	2	TTTCCACGGTCGAATAACCA	ACCTTGTTTGGACTGTATCGT	20	21	45.0	42.9	57.2	57.2	0.0	0.0	2.599817	2044	3573	1630-1636	1.000000	59.5
matK_p3	matK	3	TTTCCACGGTCGAATAACCAA	ACCTTGTTTGGACTGTATCGT	21	21	42.9	42.9	57.9	57.2	0.0	0.0	2.887394	2044	3573	1630-1636	1.000000	57.2
matK_p4	matK	4	ATTTCCACGGTCGAATAACCA	ACCTTGTTTGGACTGTATCGT	21	21	42.9	42.9	57.7	57.2	0.0	0.0	3.101931	2044	3573	1631-1637	1.000000	55.5
matK_p5	matK	5	TTCCACGGTCGAATAACCAA	CCTTGTTTGGACTGTATCGT	20	20	45.0	45.0	57.2	55.4	0.0	0.0	3.439795	2044	3573	1628-1634	1.000000	52.8
matK-trnK-UUU_p1	matK-trnK-UUU	1	GGAGGTCATGTTGCCAAGAT	TCGGGTTGCTAACTCAATGG	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.242739	3574	4335	856-856	1.000000	78.2
matK-trnK-UUU_p2	matK-trnK-UUU	2	GGAGGTCATGTTGCCAAGAT	CGGGTTGCTAACTCAATGGT	20	20	50.0	50.0	57.9	58.2	0.0	0.0	0.313573	3574	4335	855-855	1.000000	77.6
matK-trnK-UUU_p3	matK-trnK-UUU	3	TAGGAGGTCATGTTGCCAAG	TCGGGTTGCTAACTCAATGG	20	20	50.0	50.0	57.2	57.9	0.0	0.0	0.895423	3574	4335	858-858	1.000000	72.9
matK-trnK-UUU_p4	matK-trnK-UUU	4	TAGGAGGTCATGTTGCCAAG	CGGGTTGCTAACTCAATGGT	20	20	50.0	50.0	57.2	58.2	0.0	0.0	0.966257	3574	4335	857-857	1.000000	72.4
matK-trnK-UUU_p5	matK-trnK-UUU	5	AGGAGGTCATGTTGCCAAGA	TCGGGTTGCTAACTCAATGG	20	20	50.0	50.0	58.9	57.9	0.0	0.0	1.040655	3574	4335	857-857	1.000000	71.8
rps16_p1	rps16	1	AGCAGAGAAAAGATTATAATCCGA	AGTACTAACCGAACCAATGA	24	20	33.3	40.0	55.8	53.7	19.7	0.0	10.533797	5271	6421	1271-1272	1.000000	40.2
rps16_p2	rps16	2	AGCAGAGAAAAGATTATAATCCGA	AAGTACTAACCGAACCAATGA	24	21	33.3	38.1	55.8	54.6	19.7	0.0	10.648742	5271	6421	1272-1273	1.000000	40.2
rps16_p3	rps16	3	AGCAGAGAAAAGATTATAATCCGAAC	AGTACTAACCGAACCAATGA	26	20	34.6	40.0	57.6	53.7	19.7	0.0	10.661680	5271	6421	1271-1272	1.000000	40.2
rps16_p4	rps16	4	GCAGAGAAAAGATTATAATCCGAACT	AGTACTAACCGAACCAATGA	26	20	34.6	40.0	57.6	53.7	19.7	0.0	10.661680	5271	6421	1270-1271	1.000000	40.2
rps16_p5	rps16	5	GCAGAGAAAAGATTATAATCCGAACT	AAGTACTAACCGAACCAATGA	26	21	34.6	38.1	57.6	54.6	19.7	0.0	10.776625	5271	6421	1271-1272	1.000000	40.2
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	6422	7203	854-855	1.000000	78.4
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	CGGATCGTGTCCTTCAAGTC	GAGGTTCGAATCCTTCCGTC	20	20	55.0	55.0	58.4	58.1	7.8	16.9	0.445953	6422	7203	869-870	1.000000	76.5
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	GGATCGTGTCCTTCAAGTCG	GAGGTTCGAATCCTTCCGTC	20	20	55.0	55.0	58.4	58.1	2.9	16.9	0.445953	6422	7203	868-869	1.000000	76.5
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	AAGTCGCACGTTGCTTTCTA	TTCGGAGGTTCGAATCCTTC	20	20	45.0	50.0	58.1	57.3	8.1	16.9	0.806797	6422	7203	858-859	1.000000	73.6
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	AAGTCGCACGTTGCTTTCTA	TAAGGCAACGGGTTTTGGTC	20	20	45.0	50.0	58.1	58.7	8.1	0.0	0.818817	6422	7203	884-885	1.000000	73.5
trnS-GCU-trnG_p1	trnS-GCU-trnG	1	CAATCCGACGCTTTAGTCCA	ATTCATCAAACCGAGGGACC	20	20	50.0	50.0	58.0	57.6	0.0	0.0	0.424641	8586	9429	958-959	1.000000	76.9
trnS-GCU-trnG_p2	trnS-GCU-trnG	2	AATCCGACGCTTTAGTCCAC	ATTCATCAAACCGAGGGACC	20	20	50.0	50.0	58.0	57.6	0.0	0.0	0.426029	8586	9429	957-958	1.000000	76.8
trnS-GCU-trnG_p3	trnS-GCU-trnG	3	ACGGAAAGAGAGGGATTCGA	ATTCATCAAACCGAGGGACC	20	20	50.0	50.0	58.2	57.6	0.0	0.0	0.571407	8586	9429	1012-1013	1.000000	75.7
trnS-GCU-trnG_p4	trnS-GCU-trnG	4	ACGAAAACGGAAAGAGAGGG	ATTCATCAAACCGAGGGACC	20	20	50.0	50.0	57.8	57.6	0.0	0.0	0.585377	8586	9429	1018-1019	1.000000	75.6
trnS-GCU-trnG_p5	trnS-GCU-trnG	5	AGCAATCCGACGCTTTAGTC	ATTCATCAAACCGAGGGACC	20	20	50.0	50.0	58.4	57.6	0.0	0.0	0.778481	8586	9429	960-961	1.000000	74.0
rpoC1_p1	rpoC1	1	GGGACTCGAATTCACACTGG	GGAAGGACGCTTAATCGGAA	20	20	55.0	50.0	58.3	57.7	6.2	3.5	0.577898	21078	23941	2958-2958	1.000000	75.4
rpoC1_p2	rpoC1	2	GGGACTCGAATTCACACTGG	GGACGCTTAATCGGAAGGAA	20	20	55.0	50.0	58.3	57.7	6.2	3.5	0.577898	21078	23941	2954-2954	1.000000	75.4
rpoC1_p3	rpoC1	3	GGGACTCGAATTCACACTGG	AAGGACGCTTAATCGGAAGG	20	20	55.0	50.0	58.3	57.7	6.2	3.5	0.584271	21078	23941	2956-2956	1.000000	75.4
rpoC1_p4	rpoC1	4	GGGACTCGAATTCACACTGG	AGGAAGGACGCTTAATCGGA	20	20	55.0	50.0	58.3	58.5	6.2	3.5	0.791276	21078	23941	2959-2959	1.000000	73.7
rpoC1_p5	rpoC1	5	GGGACTCGAATTCACACTGG	AGGACGCTTAATCGGAAGGA	20	20	55.0	50.0	58.3	58.5	6.2	3.5	0.791276	21078	23941	2955-2955	1.000000	73.7
psaB_p1	psaB	1	GCCTCTTCTTCTCCCTCTGA	AAGGACGTGCTGTAGGAGTA	20	20	55.0	50.0	58.1	57.8	0.0	11.4	0.351948	38033	40237	2408-2408	1.000000	77.2
psaB_p2	psaB	2	GCCTCTTCTTCTCCCTCTGA	AGGACGTGCTGTAGGAGTAA	20	20	55.0	50.0	58.1	57.8	0.0	9.5	0.351948	38033	40237	2407-2407	1.000000	77.2
psaB_p3	psaB	3	GCCTCTTCTTCTCCCTCTGA	GACGTGCTGTAGGAGTAACC	20	20	55.0	55.0	58.1	57.7	0.0	0.0	0.415618	38033	40237	2405-2405	1.000000	76.7
psaB_p4	psaB	4	GCCTCTTCTTCTCCCTCTGA	GGACGTGCTGTAGGAGTAAC	20	20	55.0	55.0	58.1	57.7	0.0	0.0	0.415618	38033	40237	2406-2406	1.000000	76.7
psaB_p5	psaB	5	TTGCCTCTTCTTCTCCCTCT	AGGACGTGCTGTAGGAGTAA	20	20	50.0	50.0	57.7	57.8	0.0	9.5	0.545256	38033	40237	2409-2409	1.000000	75.7
ycf3_p1	ycf3	1	ACACATACAAAGAGACCCGC	AATGCCCTTGTTTGTGGGAT	20	20	50.0	45.0	57.9	58.0	0.0	1.8	0.123324	43187	45200	2737-2738	1.000000	79.0
ycf3_p2	ycf3	2	ACACATACAAAGAGACCCGC	ATGCCCTTGTTTGTGGGATT	20	20	50.0	45.0	57.9	58.0	0.0	1.8	0.123324	43187	45200	2736-2737	1.000000	79.0
ycf3_p3	ycf3	3	CATACAAAGAGACCCGCCAA	AATGCCCTTGTTTGTGGGAT	20	20	50.0	45.0	57.9	58.0	0.0	1.8	0.136511	43187	45200	2734-2735	1.000000	78.9
ycf3_p4	ycf3	4	CATACAAAGAGACCCGCCAA	ATGCCCTTGTTTGTGGGATT	20	20	50.0	45.0	57.9	58.0	0.0	1.8	0.136511	43187	45200	2733-2734	1.000000	78.9
ycf3_p5	ycf3	5	ACACATACAAAGAGACCCGC	ACAAAACGGGCGGTTATTCT	20	20	50.0	45.0	57.9	58.1	0.0	11.0	0.194517	43187	45200	2886-2887	1.000000	78.5
accD_p1	accD	1	AGCAAAACGATACTACGGGT	TCGTGTTTTGACTTTGATTCCT	20	22	45.0	36.4	57.0	56.5	0.0	0.0	4.554988	57247	58740	1711-1712	1.000000	43.6
accD_p2	accD	2	AGCAAAACGATACTACGGGTT	TCGTGTTTTGACTTTGATTCCT	21	22	42.9	36.4	57.7	56.5	0.0	0.0	4.834086	57247	58740	1711-1712	1.000000	41.3
accD_p3	accD	3	AGCAAAACGATACTACGGGT	TTCGTGTTTTGACTTTGATTCCT	20	23	45.0	34.8	57.0	57.2	0.0	0.0	4.872953	57247	58740	1712-1713	1.000000	41.0
accD_p4	accD	4	AGCAAAACGATACTACGGGT	AGCGAAATCACTATTTTTCACCA	20	23	45.0	34.8	57.0	57.0	0.0	0.0	5.002496	57247	58740	1680-1681	1.000000	40.0
accD_p5	accD	5	AGCAAAACGATACTACGGGT	TCGTGTTTTGACTTTGATTCC	20	21	45.0	38.1	57.0	55.0	0.0	0.0	5.038178	57247	58740	1711-1712	1.000000	40.0
accD-psaI_p1	accD-psaI	1	GGCGTTCTGGGTGAGTTATT	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	57.9	58.0	0.0	26.9	0.136428	58741	59548	947-947	1.000000	79.1
accD-psaI_p2	accD-psaI	2	GGCGTTCTGGGTGAGTTATT	TTGCCGGAAATACTAAGCCC	20	20	50.0	50.0	57.9	58.0	0.0	5.0	0.156426	58741	59548	938-938	1.000000	78.9
accD-psaI_p3	accD-psaI	3	GGCGTTCTGGGTGAGTTATT	AGAAACCATTGCAATTGCCG	20	20	50.0	45.0	57.9	57.9	0.0	26.9	0.192591	58741	59548	952-952	1.000000	78.6
accD-psaI_p4	accD-psaI	4	TAAAAGGCGTTCTGGGTGAG	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	57.8	58.0	0.0	26.9	0.205915	58741	59548	952-952	1.000000	78.5
accD-psaI_p5	accD-psaI	5	TAAAAGGCGTTCTGGGTGAG	TTGCCGGAAATACTAAGCCC	20	20	50.0	50.0	57.8	58.0	0.0	5.0	0.225914	58741	59548	943-943	1.000000	78.4
psbE-petL_p1	psbE-petL	1	CAACCCGCAATGAATAGGGA	AGCTGCTAGTAGAAAACCGA	20	20	50.0	45.0	57.9	56.3	6.4	12.5	1.763328	64900	66018	1265-1265	1.000000	66.0
psbE-petL_p2	psbE-petL	2	AGAACGTTCTCCTGTGCTTC	AGCTGCTAGTAGAAAACCGA	20	20	50.0	45.0	57.8	56.3	24.9	12.5	1.868337	64900	66018	1188-1188	1.000000	65.1
psbE-petL_p3	psbE-petL	3	ACGAATAACCAACCCGCAAT	AGCTGCTAGTAGAAAACCGA	20	20	45.0	45.0	58.2	56.3	0.0	12.5	1.883014	64900	66018	1274-1274	1.000000	65.0
psbE-petL_p4	psbE-petL	4	TGCTGACGAATAACCAACCC	AGCTGCTAGTAGAAAACCGA	20	20	50.0	45.0	58.2	56.3	0.0	12.5	1.895919	64900	66018	1279-1279	1.000000	64.9
psbE-petL_p5	psbE-petL	5	CGAATAACCAACCCGCAATG	AGCTGCTAGTAGAAAACCGA	20	20	50.0	45.0	57.8	56.3	0.0	12.5	1.899345	64900	66018	1273-1273	1.000000	64.9
rpl16-rps3_p1	rpl16-rps3	1	TCGCCCTGCTTCTATTTGTC	CCCTACAAACCATTCGAGCT	20	20	50.0	50.0	58.0	57.6	0.0	0.0	0.427427	81736	82876	1349-1350	1.000000	76.7
rpl16-rps3_p2	rpl16-rps3	2	CGCCCTGCTTCTATTTGTCT	CCCTACAAACCATTCGAGCT	20	20	50.0	50.0	58.0	57.6	0.0	0.0	0.433090	81736	82876	1348-1349	1.000000	76.7
rpl16-rps3_p3	rpl16-rps3	3	CTAGATGTGATCCAAGCGGG	CCCTACAAACCATTCGAGCT	20	20	55.0	50.0	57.8	57.6	0.0	0.0	0.564074	81736	82876	1330-1331	1.000000	75.6
rpl16-rps3_p4	rpl16-rps3	4	TCGCCCTGCTTCTATTTGTC	CCCCTACAAACCATTCGAGC	20	20	50.0	55.0	58.0	58.6	0.0	0.0	0.644649	81736	82876	1350-1351	1.000000	75.0
rpl16-rps3_p5	rpl16-rps3	5	CGCCCTGCTTCTATTTGTCT	CCCCTACAAACCATTCGAGC	20	20	50.0	55.0	58.0	58.6	0.0	0.0	0.650312	81736	82876	1349-1350	1.000000	74.9
ndhF-rpl32_p1	ndhF-rpl32	1	CCACATACGGCGCAAATTTT	TGCTGCCCAATATCCTTTCT	20	20	45.0	45.0	58.0	56.9	10.8	0.0	1.140675	112295	112667	557-577	1.000000	73.7
ndhF-rpl32_p2	ndhF-rpl32	2	CCACATACGGCGCAAATTTT	TGCTGCCCAATATCCTTTCTT	20	21	45.0	42.9	58.0	57.6	10.8	0.0	1.397502	112295	112667	557-577	1.000000	71.6
ndhF-rpl32_p3	ndhF-rpl32	3	CCACATACGGCGCAAATTTT	AAAGCTTTTAATGCTGCCCA	20	20	45.0	40.0	58.0	56.5	10.8	5.7	1.530412	112295	112667	568-588	1.000000	70.5
ndhF-rpl32_p4	ndhF-rpl32	4	CCACATACGGCGCAAATTTT	AAGCTTTTAATGCTGCCCAA	20	20	45.0	40.0	58.0	56.5	10.8	6.1	1.530412	112295	112667	567-587	1.000000	70.5
ndhF-rpl32_p5	ndhF-rpl32	5	CCACATACGGCGCAAATTTT	ATGCTGCCCAATATCCTTTCT	20	21	45.0	42.9	58.0	57.4	10.8	0.0	1.618060	112295	112667	558-578	1.000000	69.9
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	AGAAAGGATATTGGGCAGCA	AGCGTGTCTACCGATTTCAC	20	20	45.0	50.0	56.9	58.0	0.0	0.0	1.137936	112827	113630	928-936	1.000000	71.5
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	AGAAAGGATATTGGGCAGCA	CAGCGTGTCTACCGATTTCA	20	20	45.0	50.0	56.9	58.0	0.0	0.0	1.139306	112827	113630	929-937	1.000000	71.5
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	AGAAAGGATATTGGGCAGCA	TCCTAAGAGCAGCGTGTCTA	20	20	45.0	50.0	56.9	57.9	0.0	0.0	1.254382	112827	113630	938-946	1.000000	70.6
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	AGAAAGGATATTGGGCAGCA	CCTAAGAGCAGCGTGTCTAC	20	20	45.0	55.0	56.9	57.8	0.0	0.0	1.326400	112827	113630	937-945	1.000000	70.0
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	AGAAAGGATATTGGGCAGCA	CTAAGAGCAGCGTGTCTACC	20	20	45.0	55.0	56.9	57.8	0.0	0.0	1.326400	112827	113630	936-944	1.000000	70.0
ndhD_p1	ndhD	1	AGTGTACAAAACCAACAAACT	CAAAAACCCGCGCTCAAAAT	21	20	33.3	45.0	54.1	58.5	0.0	5.6	5.443971	115005	116507	1633-1636	1.000000	40.0
ndhD_p2	ndhD	2	AGTGTACAAAACCAACAAACT	TTTATCGACAAAAACCCGCG	21	20	33.3	45.0	54.1	57.4	0.0	0.0	5.538905	115005	116507	1641-1644	1.000000	40.0
ndhD_p3	ndhD	3	AAGTGTACAAAACCAACAAACT	CAAAAACCCGCGCTCAAAAT	22	20	31.8	45.0	54.9	58.5	0.0	5.6	5.617160	115005	116507	1634-1637	1.000000	40.0
ndhD_p4	ndhD	4	AGTGTACAAAACCAACAAACTT	CAAAAACCCGCGCTCAAAAT	22	20	31.8	45.0	54.9	58.5	0.0	5.6	5.617160	115005	116507	1633-1636	1.000000	40.0
ndhD_p5	ndhD	5	AGTGTACAAAACCAACAAACTT	TTTATCGACAAAAACCCGCG	22	20	31.8	45.0	54.9	57.4	0.0	0.0	5.712095	115005	116507	1641-1644	1.000000	40.0
trnR-ACG_p1	trnR-ACG	1	ACGCCCAAATTCCATAGCTT	AGGCGCTTTCACATCTTCTT	20	20	45.0	45.0	57.9	57.8	0.0	7.6	0.335003	129917	129990	260-260	1.000000	77.6
trnR-ACG_p2	trnR-ACG	2	AAGGCCATTCCATTTCGACA	AGGCGCTTTCACATCTTCTT	20	20	45.0	45.0	57.8	57.8	5.0	7.6	0.405504	129917	129990	288-288	1.000000	77.0
trnR-ACG_p3	trnR-ACG	3	AGGCCATTCCATTTCGACAA	AGGCGCTTTCACATCTTCTT	20	20	45.0	45.0	57.8	57.8	5.0	7.6	0.405504	129917	129990	287-287	1.000000	77.0
trnR-ACG_p4	trnR-ACG	4	ACGCCCAAATTCCATAGCTT	AGAGGCGCTTTCACATCTTC	20	20	45.0	50.0	57.9	58.3	0.0	7.6	0.412156	129917	129990	262-262	1.000000	77.0
trnR-ACG_p5	trnR-ACG	5	ACGCCCAAATTCCATAGCTT	GAGGCGCTTTCACATCTTCT	20	20	45.0	50.0	57.9	58.3	0.0	7.6	0.412156	129917	129990	261-261	1.000000	77.0